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Mammalian lectin arrays for screening host–microbe interactions

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J. Biol. Chem.-2020-Jégouzo-4541-55.pdfPublished version1.02 MBAdobe PDFView/Open
Title: Mammalian lectin arrays for screening host–microbe interactions
Authors: Jégouzo, SAF
Nelson, C
Hardwick, T
Wong, STA
Lau, NKK
Neoh, GKE
Castellanos-Rueda, R
Huang, Z
Mignot, B
Hirdaramani, A
Howitt, A
Frewin, K
Shen, Z
Fox, RJ
Wong, R
Ando, M
Emony, L
Zhu, H
Holder, A
Werling, D
Krishnan, N
Robertson, BD
Clements, A
Taylor, ME
Drickamer, K
Item Type: Journal Article
Abstract: Many members of the C-type lectin family of glycan-binding receptors have been ascribed roles in the recognition of microorganisms and serve as key receptors in the innate immune response to pathogens. Other mammalian receptors have become targets through which pathogens enter target cells. These receptor roles have often been documented with binding studies involving individual pairs of receptors and microorganisms. To provide a systematic overview of interactions between microbes and the large complement of C-type lectins, here we developed a lectin array and suitable protocols for labeling of microbes that could be used to probe this array. The array contains C-type lectins from cow, chosen as a model organism of agricultural interest for which the relevant pathogen–receptor interactions have not been previously investigated in detail. Screening with yeast cells and various strains of both Gram-positive and -negative bacteria revealed distinct binding patterns, which in some cases could be explained by binding to lipopolysaccharides or capsular polysaccharides, but in other cases they suggested the presence of novel glycan targets on many of the microorganisms. These results are consistent with interactions previously ascribed to the receptors, but they also highlight binding to additional sugar targets that have not previously been recognized. Our findings indicate that mammalian lectin arrays represent unique discovery tools for identifying both novel ligands and new receptor functions.
Issue Date: 3-Apr-2020
Date of Acceptance: 24-Feb-2020
URI: http://hdl.handle.net/10044/1/78165
DOI: 10.1074/jbc.RA120.012783
ISSN: 0021-9258
Publisher: American Society for Biochemistry and Molecular Biology
Start Page: 4541
End Page: 4555
Journal / Book Title: Journal of Biological Chemistry
Volume: 295
Copyright Statement: © 2020 Jégouzo et al. Author's Choice—Final version open access under the terms of the Creative Commons CC-BY license (https://creativecommons.org/licenses/by/4.0/)
Sponsor/Funder: Biotechnology and Biological Sciences Research Council (BBSRC)
Funder's Grant Number: BB/P005659/1
Keywords: Science & Technology
Life Sciences & Biomedicine
Biochemistry & Molecular Biology
glycobiology
lectin
carbohydrate-binding protein
carbohydrate function
lipopolysaccharide (LPS)
array screening
glycan-binding receptors
innate immune system
ligand binding
pathogen
C-TYPE LECTIN
CARBOHYDRATE-RECOGNITION
MOLECULAR-BASIS
BINDING
RECEPTOR
EXPRESSION
IDENTIFICATION
MECHANISM
LIPOPOLYSACCHARIDES
POLYSACCHARIDE
array screening
carbohydrate function
carbohydrate-binding protein
glycan-binding receptors
glycobiology
innate immune system
lectin
ligand binding
lipopolysaccharide (LPS)
pathogen
Amino Acid Sequence
Animals
Cattle
Gram-Negative Bacteria
Gram-Positive Bacteria
Host-Pathogen Interactions
Lectins, C-Type
Lipopolysaccharides
Polysaccharides, Bacterial
Protein Array Analysis
Saccharomyces cerevisiae
Sequence Alignment
Animals
Cattle
Gram-Negative Bacteria
Gram-Positive Bacteria
Saccharomyces cerevisiae
Lipopolysaccharides
Polysaccharides, Bacterial
Lectins, C-Type
Protein Array Analysis
Sequence Alignment
Amino Acid Sequence
Host-Pathogen Interactions
03 Chemical Sciences
06 Biological Sciences
11 Medical and Health Sciences
Biochemistry & Molecular Biology
Publication Status: Published
Online Publication Date: 2020-02-24
Appears in Collections:Department of Infectious Diseases
Faculty of Medicine
Faculty of Natural Sciences