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16S rRNA assessment of the influence of shading on early-successional biofilms in experimental streams
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16S rRNA assessment of the influence of shading on early-successional biofilms in experimental streams.pdf | Published version | 1.84 MB | Adobe PDF | View/Open |
Title: | 16S rRNA assessment of the influence of shading on early-successional biofilms in experimental streams |
Authors: | Lehmann, K Singer, A Bowes, MJ Ings, NL Field, D Bell, T |
Item Type: | Journal Article |
Abstract: | Elevated nutrient levels can lead to excessive biofilm growth, but reducing nutrient pollution is often challenging. There is therefore interest in developing control measures for biofilm growth in nutrient-rich rivers that could act as complement to direct reductions in nutrient load. Shading of rivers is one option that can mitigate blooms, but few studies have experimentally examined the differences in biofilm communities grown under shaded and unshaded conditions. We investigated the assembly and diversity of biofilm communities using in situ mesocosms within the River Thames (UK). Biofilm composition was surveyed by 454 sequencing of 16S amplicons (∼400 bp length covering regions V6/V7). The results confirm the importance of sunlight for biofilm community assembly; a resource that was utilized by a relatively small number of dominant taxa, leading to significantly less diversity than in shaded communities. These differences between unshaded and shaded treatments were either because of differences in resource utilization or loss of diatom-structures as habitats for bacteria. We observed more co-occurrence patterns and network interactions in the shaded communities. This lends further support to the proposal that increased river shading can help mitigate the effects from macronutrient pollution in rivers. |
Issue Date: | 1-Dec-2015 |
Date of Acceptance: | 19-Oct-2015 |
URI: | http://hdl.handle.net/10044/1/77719 |
DOI: | 10.1093/femsec/fiv129 |
ISSN: | 0168-6496 |
Publisher: | Oxford University Press (OUP) |
Start Page: | 1 |
End Page: | 11 |
Journal / Book Title: | FEMS Microbiology Ecology |
Volume: | 91 |
Issue: | 12 |
Copyright Statement: | © FEMS 2015. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
Sponsor/Funder: | The Royal Society |
Funder's Grant Number: | UF090325 |
Keywords: | Science & Technology Life Sciences & Biomedicine Microbiology biofilm diversity riparian shading biofilm composition networks RIPARIAN VEGETATION LIGHT LIMITATION WATER PHOSPHORUS RIVER NUTRIENTS COMMUNITY PHYTOPLANKTON PERIPHYTON ALGAE biofilm composition biofilm diversity networks riparian shading Bacteria Base Sequence Biofilms Diatoms Ecosystem England Eutrophication Molecular Typing RNA, Ribosomal, 16S Rivers Sequence Analysis, RNA Sunlight Water Microbiology Diatoms Bacteria Biofilms RNA, Ribosomal, 16S Sequence Analysis, RNA Water Microbiology Ecosystem Sunlight Rivers Eutrophication Base Sequence England Molecular Typing Science & Technology Life Sciences & Biomedicine Microbiology biofilm diversity riparian shading biofilm composition networks RIPARIAN VEGETATION LIGHT LIMITATION WATER PHOSPHORUS RIVER NUTRIENTS COMMUNITY PHYTOPLANKTON PERIPHYTON ALGAE Microbiology 05 Environmental Sciences 06 Biological Sciences 11 Medical and Health Sciences |
Publication Status: | Published |
Article Number: | ARTN fiv129 |
Online Publication Date: | 2015-10-23 |
Appears in Collections: | Grantham Institute for Climate Change Faculty of Natural Sciences |