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B cell diversification Is uncoupled from SAP-mediated selection forces in chronic germinal centers within Peyer's patches

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Title: B cell diversification Is uncoupled from SAP-mediated selection forces in chronic germinal centers within Peyer's patches
Authors: Biram, A
Winter, E
Denton, AE
Zaretsky, I
Dassa, B
Bemark, M
Linterman, MA
Yaari, G
Shulman, Z
Item Type: Journal Article
Abstract: Antibodies secreted within the intestinal tract provide protection from the invasion of microbes into the host tissues. Germinal center (GC) formation in lymph nodes and spleen strictly requires SLAM-associated protein (SAP)-mediated T cell functions; however, it is not known whether this mechanism plays a similar role in mucosal-associated lymphoid tissues. Here, we find that in Peyer's patches (PPs), SAP-mediated T cell help is required for promoting B cell selection in GCs, but not for clonal diversification. PPs of SAP-deficient mice host chronic GCs that are absent in T cell-deficient mice. GC B cells in SAP-deficient mice express AID and Bcl6 and generate plasma cells in proportion to the GC size. Single-cell IgA sequencing analysis reveals that these mice host few diversified clones that were subjected to mild selection forces. These findings demonstrate that T cell-derived help to B cells in PPs includes SAP-dependent and SAP-independent functions.
Issue Date: 11-Feb-2020
Date of Acceptance: 8-Jan-2020
URI: http://hdl.handle.net/10044/1/77266
DOI: 10.1016/j.celrep.2020.01.032
ISSN: 2211-1247
Publisher: Elsevier
Start Page: 1910
End Page: 1922.e5
Journal / Book Title: Cell Reports
Volume: 30
Issue: 6
Copyright Statement: © 2020 The Authors.This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/)
Keywords: B cells
IgA
Peyer’s patches
SAP
T follicular helper cells
antibody
clonal diversification
germinal center
plasma cells
B cells
IgA
Peyer’s patches
SAP
T follicular helper cells
antibody
clonal diversification
germinal center
plasma cells
0601 Biochemistry and Cell Biology
1116 Medical Physiology
Publication Status: Published
Conference Place: United States
Open Access location: https://www.sciencedirect.com/science/article/pii/S2211124720300474
Online Publication Date: 2020-02-11
Appears in Collections:Department of Immunology and Inflammation