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Replicating landmine blast loading in cellular in Vitro models

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Sory_2020_Phys._Biol._17_056001.pdfPublished version5.94 MBAdobe PDFView/Open
Sory+et+al_2020_Phys._Biol._10.1088_1478-3975_ab7d1c_suppl.pdfSupporting information1.39 MBAdobe PDFView/Open
Title: Replicating landmine blast loading in cellular <i>in Vitro</i> models
Authors: Sory, DR
Amin, HD
Chapman, D
Proud, WG
Rankin, SM
Item Type: Journal Article
Abstract: Trauma arising from landmines and improvised explosive devices promotes heterotopic ossification, the formation of extra-skeletal bone in non-osseous tissue. To date, experimental platforms that can replicate the loading parameter space relevant to improvised explosive device and landmine blast wave exposure have not been available to study the effects of such non-physiological mechanical loading on cells. Here, we present the design and calibration of three distinct in vitro experimental loading platforms that allow us to replicate the spectrum of loading conditions recorded in near-field blast wave exposure. We subjected cells in suspension or in a three-dimensional hydrogel to strain rates up to 6000 s-1and pressure levels up to 45 MPa. Our results highlight that cellular activation is regulated in a non-linear fashion - not by a single mechanical parameter, it is the combined action of the applied mechanical pressure, rate of loading and loading impulse, along with the extracellular environment used to convey the pressure waves. Finally, our research indicates that PO MSCs are finely tuned to respond to mechanical stimuli that fall within defined ranges of loading.
Issue Date: 1-Sep-2020
Date of Acceptance: 5-Mar-2020
URI: http://hdl.handle.net/10044/1/77236
DOI: 10.1088/1478-3975/ab7d1c
ISSN: 1478-3967
Publisher: IOP Publishing
Journal / Book Title: Physical Biology
Volume: 17
Issue: 5
Copyright Statement: s © 2020 The Author(s). Published by IOP Publishing Ltd. As the Version of Record of this article is going to be / has been published on a gold open access basis under a CC BY 3.0 licence, this Accepted Manuscript is available for reuse under a CC BY 3.0 licence immediately. Everyone is permitted to use all or part of the original content in this article, provided that they adhere to all the terms of the licence https://creativecommons.org/licences/by/3.0
Sponsor/Funder: The Royal British Legion
Funder's Grant Number: BMPF_P60304
Keywords: anti personnel landmine
blast injuries
split-Hopkinson pressure bar
stem cell
strain rate
02 Physical Sciences
06 Biological Sciences
09 Engineering
Publication Status: Published
Conference Place: England
Online Publication Date: 2020-03-05
Appears in Collections:Physics
National Heart and Lung Institute
Plasma Physics
Faculty of Medicine
Faculty of Natural Sciences