8
IRUS Total
Downloads
  Altmetric

H3 lysine 4 8 is acetylated at active gene promoters and is regulated by H3 lysine 4 methylation

Title: H3 lysine 4 8 is acetylated at active gene promoters and is regulated by H3 lysine 4 methylation
Authors: Guillemette, B
Drogaris, P
Lin, H-HS
Armstrong, H
Hiragami-Hamada, K
Imhof, A
Bonneil, E
Thibault, P
Verreault, A
Festenstein, RJ
Item Type: Journal Article
Abstract: Methylation of histone H3 lysine 4 (H3K4me) is an evolutionarily conserved modification whose role in the regulation of gene expression has been extensively studied. In contrast, the function of H3K4 acetylation (H3K4ac) has received little attention because of a lack of tools to separate its function from that of H3K4me. Here we show that, in addition to being methylated, H3K4 is also acetylated in budding yeast. Genetic studies reveal that the histone acetyltransferases (HATs) Gcn5 and Rtt109 contribute to H3K4 acetylation in vivo. Whilst removal of H3K4ac from euchromatin mainly requires the histone deacetylase (HDAC) Hst1, Sir2 is needed for H3K4 deacetylation in heterochomatin. Using genome-wide chromatin immunoprecipitation (ChIP), we show that H3K4ac is enriched at promoters of actively transcribed genes and located just upstream of H3K4 tri-methylation (H3K4me3), a pattern that has been conserved in human cells. We find that the Set1-containing complex (COMPASS), which promotes H3K4me2 and -me3, also serves to limit the abundance of H3K4ac at gene promoters. In addition, we identify a group of genes that have high levels of H3K4ac in their promoters and are inadequately expressed in H3-K4R, but not in set1Δ mutant strains, suggesting that H3K4ac plays a positive role in transcription. Our results reveal a novel regulatory feature of promoter-proximal chromatin, involving mutually exclusive histone modifications of the same histone residue (H3K4ac and H3K4me).
Issue Date: 1-Mar-2011
Date of Acceptance: 25-Feb-2011
URI: http://hdl.handle.net/10044/1/77203
DOI: 10.1371/journal.pgen.1001354
ISSN: 1553-7390
Publisher: Public Library of Science (PLoS)
Start Page: 1
End Page: 16
Journal / Book Title: PLoS Genetics
Volume: 7
Issue: 3
Copyright Statement: © 2011 Guillemette et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Sponsor/Funder: Medical Research Council (MRC)
Medical Research Council (MRC)
Funder's Grant Number: G0301153
G116/182
Keywords: Science & Technology
Life Sciences & Biomedicine
Genetics & Heredity
GLOBAL HISTONE ACETYLATION
SACCHAROMYCES-CEREVISIAE
PHD FINGER
GENOME-WIDE
AMINO-ACID
PLANT HOMEODOMAIN
COMPLEX INCLUDES
DNA-REPLICATION
SET1 COMPLEX
YEAST
Acetylation
Euchromatin
Gene Expression Regulation, Enzymologic
Gene Regulatory Networks
Heterochromatin
Histone Acetyltransferases
Histone-Lysine N-Methyltransferase
Histones
Lysine
Methylation
Promoter Regions, Genetic
Saccharomyces cerevisiae
Saccharomyces cerevisiae Proteins
Silent Information Regulator Proteins, Saccharomyces cerevisiae
Sirtuin 2
Euchromatin
Heterochromatin
Saccharomyces cerevisiae
Histone-Lysine N-Methyltransferase
Lysine
Saccharomyces cerevisiae Proteins
Silent Information Regulator Proteins, Saccharomyces cerevisiae
Histones
Gene Expression Regulation, Enzymologic
Acetylation
Methylation
Histone Acetyltransferases
Gene Regulatory Networks
Promoter Regions, Genetic
Sirtuin 2
Science & Technology
Life Sciences & Biomedicine
Genetics & Heredity
GLOBAL HISTONE ACETYLATION
SACCHAROMYCES-CEREVISIAE
PHD FINGER
GENOME-WIDE
AMINO-ACID
PLANT HOMEODOMAIN
COMPLEX INCLUDES
DNA-REPLICATION
SET1 COMPLEX
YEAST
Developmental Biology
0604 Genetics
Publication Status: Published
Article Number: ARTN e1001354
Online Publication Date: 2011-03-31
Appears in Collections:Department of Brain Sciences