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Fiber-optic SERS probes fabricated using two-photon polymerization for rapid detection of bacteria

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Title: Fiber-optic SERS probes fabricated using two-photon polymerization for rapid detection of bacteria
Authors: Kim, JA
Wales, D
Thompson, A
Yang, G-Z
Item Type: Journal Article
Abstract: This study presents a novel fiber-optic surface-enhanced Raman spectroscopy (SERS) probe (SERS-on-a-tip) fabricated using a simple, two-step protocol based on off-the-shelf components and materials, with a high degree of controllability and repeatability. Two-photon polymerization and subsequent metallization was adopted to fabricate a range of SERS arrays on both planar substrates and end-facets of optical fibers. For the SERS-on-a-tip probes, a limit of detection of 10-7 M (Rhodamine 6G) and analytical enhancement factors of up to 1300 were obtained by optimizing the design, geometry and alignment of the SERS arrays on the tip of the optical fiber. Furthermore, strong repeatability and consistency were achieved for the fabricated SERS arrays, demonstrating that the technique may be suitable for large-scale fabrication procedures in the future. Finally, rapid SERS detection of live Escherichia coli cells was demonstrated using integration times in the milliseconds to seconds range. This result indicates strong potential for in vivo diagnostic use, particularly for detection of infections. Moreover, to the best of our knowledge, this represents the first report of detection of live, unlabeled bacteria using a fiber-optic SERS probe.
Issue Date: 4-May-2020
Date of Acceptance: 6-Jan-2020
URI: http://hdl.handle.net/10044/1/75908
DOI: 10.1002/adom.201901934
ISSN: 2195-1071
Publisher: Wiley
Start Page: 1
End Page: 12
Journal / Book Title: Advanced Optical Materials
Volume: 8
Issue: 9
Copyright Statement: © 2020 The Authors. Published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Sponsor/Funder: Engineering & Physical Science Research Council (EPSRC)
National Institutes of Health
Funder's Grant Number: EP/P012779/1
NIHR
Keywords: Science & Technology
Technology
Physical Sciences
Materials Science, Multidisciplinary
Optics
Materials Science
bacteria detection
optical fibers
Raman spectroscopy
SERS
two-photon polymerization
HIGH-PERFORMANCE SERS
RAMAN-SPECTROSCOPY
IN-VIVO
ARRAYS
NANOSTRUCTURES
FIBERS
0205 Optical Physics
0906 Electrical and Electronic Engineering
0912 Materials Engineering
Publication Status: Published
Online Publication Date: 2020-02-25
Appears in Collections:Department of Surgery and Cancer
Computing
Faculty of Medicine
Institute of Global Health Innovation
Faculty of Engineering