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Imaging and tissue biomarkers of choline metabolism in diffuse adult glioma; 18F-fluoromethylcholine PET/CT, magnetic resonance spectroscopy, and choline kinase α

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Title: Imaging and tissue biomarkers of choline metabolism in diffuse adult glioma; 18F-fluoromethylcholine PET/CT, magnetic resonance spectroscopy, and choline kinase α
Authors: Grech-Sollars, M
Ordidge, KL
Vaqas, B
Davies, C
Vaja, V
Honeyfield, L
Camp, S
Towey, D
Mayers, H
Peterson, D
O'Neill, K
Roncaroli, F
Barwick, TD
Waldman, AD
Item Type: Journal Article
Abstract: The cellular and molecular basis of choline uptake on PET imaging and MRS-visible choline containing compounds is not well understood. Choline kinase alpha (ChoKa) is an enzyme that phosphorylates choline, an essential step in membrane synthesis. We investigate choline metabolism through 18F-fluoromethylcholine (18F-FMC) PET, MRS and tissue ChoKa in human glioma. 14 patients with suspected diffuse glioma underwent multimodal 3T MRI and dynamic 18F FMC PET/CT prior to surgery. Co-registered PET and MRI data were used to target biopsies to regions of high and low choline signal, and immunohistochemistry for ChoKa expression was performed. 18F-FMC/PET differentiated WHO grade IV from grade II and III tumours, whereas MRS differentiated grade III/IV from grade II tumours. Tumoural 18F-FMC/PET uptake was higher than in normal-appearing white matter across all grades and markedly elevated within regions of contrast enhancement. 18F-FMC/PET correlated weakly with MRS Cho ratios. ChoKa expression on IHC was negative or weak in all but one GBM sample, and did not correlate with tumour grade or imaging choline markers. MRS and 18F-FMC/PET provide complimentary information on glioma choline metabolism. Tracer uptake is, however, potentially confounded by blood-brain barrier permeability. ChoKa overexpression does not appear to be a common feature in diffuse glioma.
Issue Date: 7-Dec-2019
Date of Acceptance: 3-Dec-2019
URI: http://hdl.handle.net/10044/1/75476
DOI: 10.3390/cancers11121969
ISSN: 2072-6694
Publisher: MDPI AG
Start Page: 1
End Page: 15
Journal / Book Title: Cancers
Volume: 11
Issue: 12
Copyright Statement: © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Sponsor/Funder: Imperial College Healthcare NHS Trust- BRC Funding
Imperial Health Charity
Brain Tumour Research Campaign
The Brain Tumour Charity
Funder's Grant Number: RDC04 79560
5098
n/a
33/159
Keywords: Science & Technology
Life Sciences & Biomedicine
Oncology
brain
choline
glioma
MRI
PET
PROLIFERATIVE ACTIVITY
PROTON
TUMORS
LESIONS
INDEX
MRI
PET
brain
choline
glioma
1112 Oncology and Carcinogenesis
Publication Status: Published
Article Number: ARTN 1969
Online Publication Date: 2019-12-07
Appears in Collections:Department of Surgery and Cancer
Department of Medicine (up to 2019)
Faculty of Medicine
Department of Brain Sciences