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Passage of influenza A/H3N2 viruses in human airway cells removes artefactual variants associated with neuraminidase-mediated binding.

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Title: Passage of influenza A/H3N2 viruses in human airway cells removes artefactual variants associated with neuraminidase-mediated binding.
Authors: Brown, JC
Barclay, WS
Galiano, M
Harvey, R
Item Type: Journal Article
Abstract: Serological assays with modern influenza A/H3N2 viruses have become problematic due to the progressive reduction in the ability of viruses of this subtype to bind and agglutinate red blood cells (RBCs). This is due to reduced ability of the viral haemagglutinin (HA) glycoprotein to bind to the sialic acid-containing receptors presented by these cells. Additionally, as a result of reduced HA-mediated binding in cell culture, modern A/H3N2 viruses often acquire compensatory mutations during propagation that enable binding of cellular receptors through their neuraminidase (NA) surface protein. Viruses that have acquired this NA-mediated binding agglutinate RBCs through their NA, confusing the results of serological assays designed to assess HA antigenicity. Here we confirm with a large dataset that the acquisition of mutations that confer NA binding of RBCs is a culture artefact, and demonstrate that modern A/H3N2 isolates with acquired NA-binding mutations revert to a clinical-like NA sequence after a single passage in human airway epithelial (HAE) cells.
Issue Date: 8-Nov-2019
Date of Acceptance: 11-Oct-2019
URI: http://hdl.handle.net/10044/1/74959
DOI: 10.1099/jgv.0.001348
ISSN: 0022-1317
Publisher: Microbiology Society
Journal / Book Title: Journal of General Virology
Copyright Statement: © 2019 Not applicable. This is an open-access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/deed.ast).
Keywords: H3N2
haemagglutination
influenza
neuraminidase
serology
vaccine
H3N2
haemagglutination
influenza
neuraminidase
serology
vaccine
06 Biological Sciences
07 Agricultural and Veterinary Sciences
11 Medical and Health Sciences
Virology
Publication Status: Published online
Conference Place: England
Open Access location: https://doi.org/10.1099/jgv.0.001348
Online Publication Date: 2019-11-08
Appears in Collections:Faculty of Medicine



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