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Metabolic phenotyping in venous disease: The need for standardization

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Title: Metabolic phenotyping in venous disease: The need for standardization
Authors: Onida, S
Tan, MKH
Kafeza, M
Bergner, RT
Shalhoub, J
Holmes, E
Davies, AH
Item Type: Journal Article
Abstract: Venous thromboembolism (VTE), chronic venous disease (CVD), and venous leg ulceration (VLU) are clinical manifestations of a poorly functioning venous system. Though common, much is unknown of the pathophysiology and progression of these conditions. Metabolic phenotyping has been employed to explore mechanistic pathways involved in venous disease. A systematic literature review was performed: full text, primary research articles on the applications of nuclear magnetic resonance spectroscopy (NMR) and mass spectrometry (MS) in human participants and animals were included for qualitative synthesis. Seventeen studies applying metabolic phenotyping to venous disease were identified: six on CVD, two on VLU, and nine on VTE; both animal (n = 6) and human (n = 10) experimental designs were reported, with one study including both. NMR, MS, and MS imaging were employed to characterize serum, plasma, urine, wound fluid, and tissue. Metabolites found to be upregulated in CVD included lipids, branched chain amino acids (BCAA), glutamate, taurine, lactate, and myo-inositol identified in vein tissue. Upregulated metabolites in VLU included lactate, BCAA, lysine, 3-hydroxybutyrate, and glutamate identified in wound fluid and ulcer biopsies. VTE cases were associated with reduced carnitine levels, upregulated aromatic amino acids, 3-hydroxybutyrate, BCAA, and lipids in plasma, serum, thrombus, and vein wall; kynurenine and tricarboxylic acid pathway dysfunction were reported. Future research should focus on targeted studies with internal and external validation.
Issue Date: 1-Nov-2019
Date of Acceptance: 3-Oct-2019
URI: http://hdl.handle.net/10044/1/74119
DOI: 10.1021/acs.jproteome.9b00460
ISSN: 1535-3893
Publisher: American Chemical Society
Start Page: 3809
End Page: 3820
Journal / Book Title: Journal of Proteome Research
Volume: 18
Issue: 11
Copyright Statement: © 2019 American Chemical Society. This document is the Accepted Manuscript version of a Published Work that appeared in final form in Journal of Proteome Research, after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/acs.jproteome.9b00460.
Keywords: biomarker
chronic venous disease
metabolic phenotyping
metabolomics
metabonomics
omics
systems biology
venous leg ulceration
venous thromboembolism
biomarker
chronic venous disease
metabolic phenotyping
metabolomics
metabonomics
omics
systems biology
venous leg ulceration
venous thromboembolism
06 Biological Sciences
03 Chemical Sciences
Biochemistry & Molecular Biology
Publication Status: Published
Conference Place: United States
Online Publication Date: 2019-10-16
Appears in Collections:Department of Surgery and Cancer