Microscopic viscosity of neuronal plasma membranes measured using fluorescent molecular rotors: effects of oxidative stress and neuroprotection.

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Title: Microscopic viscosity of neuronal plasma membranes measured using fluorescent molecular rotors: effects of oxidative stress and neuroprotection.
Authors: Kubankova, M
Summers, P
Lopez-Duarte, I
Kiryushko, D
Kuimova, MK
Item Type: Journal Article
Abstract: Molecular mobility in neuronal plasma membranes is a crucial factor in brain function. Microscopic viscosity is an important parameter that determines molecular mobility. This study presents the first direct measurements of the microviscosity of plasma membranes of live neurons. Microviscosity maps were obtained using fluorescence lifetime imaging of environment-sensing dyes termed 'molecular rotors'. Neurons were investigated both in the basal state and following common neurodegenerative stimuli, excitotoxicity or oxidative stress. Both types of neurotoxic challenges induced microviscosity decrease in cultured neurons, and the oxidant-induced membrane fluidification was counteracted by the wide-spectrum neuroprotectant, the H3 peptide. These results provide new insights into molecular mobility in neuronal membranes, paramount for basic brain function, and suggest that preservation of membrane stability may be an important aspect of neuroprotection in brain insults and neurodegenerative disorders.
Issue Date: 9-Oct-2019
Date of Acceptance: 12-Sep-2019
URI: http://hdl.handle.net/10044/1/73521
DOI: https://doi.org/10.1021/acsami.9b10426
ISSN: 1944-8244
Publisher: American Chemical Society
Start Page: 36307
End Page: 36315
Journal / Book Title: ACS Applied Materials and Interfaces
Volume: 11
Issue: 40
Copyright Statement: © 2019 American Chemical Society. This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Applied Materials and Interfaces, after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/acsami.9b10426
Sponsor/Funder: Commission of the European Communities
Engineering & Physical Science Research Council (EPSRC)
Engineering & Physical Science Research Council (EPSRC)
Engineering & Physical Science Research Council (EPSRC)
Funder's Grant Number: PIEF-GA-2013-627049
EP/I003983/1
EP/K030760/1
n/a
Keywords: H3 peptide
excitotoxicity
fluorescence lifetime imaging
microviscosity
molecular rotor
neurons
neuroprotection
oxidative stress
plasma membrane
0904 Chemical Engineering
0303 Macromolecular and Materials Chemistry
0306 Physical Chemistry (incl. Structural)
Nanoscience & Nanotechnology
Publication Status: Published
Conference Place: United States
Embargo Date: 2020-09-12
Online Publication Date: 2019-09-12
Appears in Collections:Physics
Chemistry
Experimental Solid State
Faculty of Natural Sciences



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