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Gene expression in granulosa cells from small antral follicles from women with or without polycystic ovaries

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Title: Gene expression in granulosa cells from small antral follicles from women with or without polycystic ovaries
Authors: Owens, LA
Kristensen, SG
Lerner, A
Christopoulos, G
Lavery, S
Hanyaloglu, AC
Hardy, K
Yding Andersen, C
Franks, S
Item Type: Journal Article
Abstract: CONTEXT: Polycystic ovary syndrome (PCOS) is the commonest cause of anovulation. A key feature of PCOS is arrest of follicles at the small-medium sized antral stage. OBJECTIVE AND DESIGN: To provide further insight into the mechanism of follicle arrest in PCOS, we profiled; (1) gonadotropin receptors; (2) characteristics of aberrant steroidogenesis, and (3) expression of anti-Mullerian hormone (AMH) and its receptor in granulosa cells (GCs) from unstimulated, human small antral follicles (hSAFs) and from granulosa-lutein cells (GLCs). SETTING: GCs from hSAFs were collected at the time of cryopreservation of ovarian tissue for fertility preservation and GLCs collected during oocyte aspiration before IVF/ICSI. PARTICIPANTS: hSAF GCs were collected from 31 women (98 follicles), 10 with polycystic ovaries (PCO) and 21 without. GLCs were collected from 6 women with PCOS and 6 controls undergoing IVF. MAIN OUTCOME MEASURES: Expression of the following genes: LHCGR, FSHR, AR, INSR, HSD3B2, CYP11A1, CYP19, STAR, AMH, AMHR2, FST, INHBA, INHBB in GCs and GLCs were compared between women with PCO and controls. RESULTS: GCs in hSAFs from PCO women showed higher expression of LHCGR in a subset (20%) of follicles. Expression of FSHR (p<0.05), AR (p<0.05), CYP11A1 (P<0.05) was lower, and expression of CYP19A1 (p<0.05), STAR (p<0.05), HSD3B2 (ns), INHBA (p<0.05) higher in PCO GCs. Gene expression in GL cells differed between women with and without PCOS but also differed from that in GCs. CONCLUSIONS: Follicle arrest in PCO is characterised in GCs by differential regulation of key genes involved in follicle growth and function.
Issue Date: 1-Dec-2019
Date of Acceptance: 1-Jul-2019
URI: http://hdl.handle.net/10044/1/71771
DOI: 10.1210/jc.2019-00780
ISSN: 0021-972X
Publisher: Oxford University Press (OUP)
Start Page: 6182
End Page: 6192
Journal / Book Title: Journal of Clinical Endocrinology and Metabolism
Volume: 104
Issue: 12
Copyright Statement: © 2019 The Authors. This article has been published under the terms of the Creative Commons Attribution License (CC BY; https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Sponsor/Funder: Genesis Research Trust
Medical Research Council (MRC)
Funder's Grant Number: 01028
MR/M012638/1
Keywords: 1103 Clinical Sciences
1114 Paediatrics and Reproductive Medicine
Endocrinology & Metabolism
Publication Status: Published
Conference Place: United States
Online Publication Date: 2019-07-05
Appears in Collections:Faculty of Medicine



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