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F9 Fimbriae of uropathogenic Escherichia coli are expressed at low temperature and recognise Gal beta 1-3GlcNAc-containing glycans

Title: F9 Fimbriae of uropathogenic Escherichia coli are expressed at low temperature and recognise Gal beta 1-3GlcNAc-containing glycans
Authors: Wurpel, DJ
Totsika, M
Allsopp, LP
Hartley-Tassell, LE
Day, CJ
Peters, KM
Sarkar, S
Ulett, GC
Yang, J
Tiralongo, J
Strugnell, RA
Jennings, MP
Schembri, MA
Item Type: Journal Article
Abstract: Uropathogenic Escherichia coli (UPEC) is the leading causative agent of urinary tract infections (UTI) in the developed world. Among the major virulence factors of UPEC, surface expressed adhesins mediate attachment and tissue tropism. UPEC strains typically possess a range of adhesins, with type 1 fimbriae and P fimbriae of the chaperone-usher class the best characterised. We previously identified and characterised F9 as a new chaperone-usher fimbrial type that mediates biofilm formation. However, the regulation and specific role of F9 fimbriae remained to be determined in the context of wild-type clinical UPEC strains. In this study we have assessed the distribution and genetic context of the f9 operon among diverse E. coli lineages and pathotypes and demonstrated that f9 genes are significantly more conserved in a UPEC strain collection in comparison to the well-defined E. coli reference (ECOR) collection. In the prototypic UPEC strain CFT073, the global regulator protein H-NS was identified as a transcriptional repressor of f9 gene expression at 37uC through its ability to bind directly to the f9 promoter region. F9 fimbriae expression was demonstrated at 20uC, representing the first evidence of functional F9 fimbriae expression by wild-type E. coli. Finally, glycan array analysis demonstrated that F9 fimbriae recognise and bind to terminal Galb1-3GlcNAc structures.
Issue Date: 26-Mar-2014
Date of Acceptance: 3-Mar-2014
URI: http://hdl.handle.net/10044/1/71092
DOI: https://doi.org/10.1371/journal.pone.0093177
ISSN: 1932-6203
Publisher: Public Library of Science (PLoS)
Journal / Book Title: PLoS ONE
Volume: 9
Issue: 3
Copyright Statement: © 2014 Wurpel et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Keywords: Science & Technology
Multidisciplinary Sciences
Science & Technology - Other Topics
COMPLETE GENOME SEQUENCE
H-NS
MOLECULAR CHARACTERIZATION
AUTOTRANSPORTER PROTEINS
BIOFILM FORMATION
CLONING VEHICLES
S-FIMBRIAE
E.-COLI
IDENTIFICATION
COMMENSAL
Acetylglucosamine
Adhesins, Bacterial
Amino Acid Sequence
Bacterial Adhesion
Base Sequence
Biofilms
Escherichia coli Proteins
Evolution, Molecular
Fimbriae, Bacterial
Galactose
Gene Expression Regulation, Bacterial
Genes, Bacterial
Molecular Sequence Data
Operon
Phylogeny
Polysaccharides
Transcription, Genetic
Uropathogenic Escherichia coli
Fimbriae, Bacterial
Biofilms
Acetylglucosamine
Galactose
Polysaccharides
Adhesins, Bacterial
Escherichia coli Proteins
Evolution, Molecular
Phylogeny
Bacterial Adhesion
Transcription, Genetic
Gene Expression Regulation, Bacterial
Amino Acid Sequence
Base Sequence
Genes, Bacterial
Operon
Molecular Sequence Data
Uropathogenic Escherichia coli
Science & Technology
Multidisciplinary Sciences
Science & Technology - Other Topics
COMPLETE GENOME SEQUENCE
H-NS
MOLECULAR CHARACTERIZATION
AUTOTRANSPORTER PROTEINS
BIOFILM FORMATION
CLONING VEHICLES
S-FIMBRIAE
E.-COLI
IDENTIFICATION
COMMENSAL
General Science & Technology
MD Multidisciplinary
Publication Status: Published
Article Number: e93177
Online Publication Date: 2014-03-26
Appears in Collections:National Heart and Lung Institute