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Towards development of fibre-optic surface enhanced Raman spectroscopy probes using 2-photon polymerisation for rapid detection of bacteria

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Kim (2019) - Towards Fiber-optic SERS (published version).pdfPublished version1.16 MBAdobe PDFView/Open
Title: Towards development of fibre-optic surface enhanced Raman spectroscopy probes using 2-photon polymerisation for rapid detection of bacteria
Authors: Kim, JA
Wales, DJ
Thompson, AJ
Yang, G-Z
Item Type: Conference Paper
Abstract: In this study, a variety of direct laser written surface-enhanced Raman spectroscopy (SERS) micro-structures, designed for bacteria detection, are presented. Various SERS micro-structures were designed to achieve both a high density of plasmonic hot spots and a strong probability of interaction between the hot spots and the target bacterial cells. Twophoton polymerization was used for initial fabrication of the polymeric skeletons of the SERS micro-structures, which were then coated with a 50 nm-thick gold layer via e-beam evaporation. The micro-structures were fabricated on glass coverslips and were assessed using a confocal Raman microscope. To this end, Rhodamine 6G was used as an analyte under 785 nm laser illumination. The optimal SERS micro-structures showed approximately 7×103 enhancement in Raman signal (analytical enhancement factor, AEF) at a wavenumber of 600 cm-1. Real-time detection of E. coli in solution was demonstrated using the fabricated SERS platform with low laser powers and a short acquisition time (785 nm, 5 mW, 50 ms).
Issue Date: 7-Mar-2019
Date of Acceptance: 2-Feb-2019
URI: http://hdl.handle.net/10044/1/70994
DOI: https://doi.org/10.1117/12.2507961
ISBN: 9781510624306
ISSN: 0277-786X
Publisher: SPIE
Journal / Book Title: Proceedings of SPIE: Plasmonics in Biology and Medicine XVI
Copyright Statement: © 2019 SPIE.
Sponsor/Funder: Engineering & Physical Science Research Council (EPSRC)
Funder's Grant Number: EP/P012779/1
Conference Name: Plasmonics in Biology and Medicine XVI
Publication Status: Published
Start Date: 2019-02-02
Finish Date: 2019-02-07
Conference Place: San Francisco, California, United States
Online Publication Date: 2019-03-07
Appears in Collections:Department of Surgery and Cancer