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In vivo label-free optical monitoring of structural and metabolic remodeling of myocardium following infarction

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Title: In vivo label-free optical monitoring of structural and metabolic remodeling of myocardium following infarction
Authors: Lagarto, J
Dyer, B
Dunsby, C
Peters, N
French, P
Dunsby, C
Lyon, A
Item Type: Journal Article
Abstract: Cardiac remodeling following myocardial infarction (MI) involves structural and functional alterations in the infarcted and remote viable myocardium that can ultimately lead to heart failure. The underlying mechanisms are not fully understood and, following our previous study of the autofluorescence lifetime and diffuse reflectance signatures of the myocardium in vivo at 16 weeks post MI in rats [Biomed. Opt. Express 6(2), 324 (2015)], we here present data obtained at 1, 2 and 4 weeks post myocardial infarction that help follow the temporal progression of these changes. Our results demonstrate that both structural and metabolic changes in the heart can be monitored from the earliest time points following MI using label-free optical readouts, not only in the region of infarction but also in the remote non-infarcted myocardium. Changes in the autofluorescence intensity and lifetime parameters associated with collagen type I autofluorescence were indicative of progressive collagen deposition in tissue that was most pronounced at earlier time points and in the region of infarction. In addition to significant collagen deposition in infarcted and non-infarcted myocardium, we also report changes in the autofluorescence parameters associated with reduced nicotinamide adenine (phosphate) dinucleotide (NAD(P)H) and flavin adenine dinucleotide (FAD), which we associate with metabolic alterations throughout the heart. Parallel measurements of the diffuse reflectance spectra indicated an increased contribution of reduced cytochrome c. Our findings suggest that combining time-resolved spectrofluorometry and diffuse reflectance spectroscopy could provide a useful means to monitor cardiac function in vivo at the time of surgery.
Issue Date: 21-Jun-2019
Date of Acceptance: 29-May-2019
URI: http://hdl.handle.net/10044/1/70738
DOI: 10.1364/BOE.10.003506
ISSN: 2156-7085
Publisher: Optical Society of America
Start Page: 3506
End Page: 3521
Journal / Book Title: Biomedical Optics Express
Volume: 10
Issue: 7
Copyright Statement: © 2019 Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License (https://creativecommons.org/licenses/by/4.0/). Further distribution of this work must maintain attribution to the author(s) and the published article’s title, journal citation, and DOI.
Sponsor/Funder: Engineering & Physical Science Research Council (EPSRC)
British Heart Foundation
British Heart Foundation
British Heart Foundation
British Heart Foundation
Rosetrees Trust
Imperial College Healthcare NHS Trust- BRC Funding
Imperial College Healthcare NHS Trust- BRC Funding
British Heart Foundation
Funder's Grant Number: EP/I02770X/1
RG/16/3/32175
FS/11/67/28954
PG/16/17/32069
PG/16/17/32069
A1407/ M645
RDB02
RDF01
RE/18/4/34215
Keywords: Science & Technology
Life Sciences & Biomedicine
Physical Sciences
Biochemical Research Methods
Optics
Radiology, Nuclear Medicine & Medical Imaging
Biochemistry & Molecular Biology
TIME-RESOLVED AUTOFLUORESCENCE
FLUORESCENCE
SPECTROSCOPY
MATRIX
MORPHOLOGY
MYOGLOBIN
EXPANSION
CARTILAGE
TISSUES
STATES
0205 Optical Physics
0912 Materials Engineering
Publication Status: Published
Online Publication Date: 2019-06-21
Appears in Collections:Physics
Photonics
National Heart and Lung Institute
Faculty of Natural Sciences