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Detection of proteoglycan loss from articular cartilage using Brillouin microscopy, with applications to osteoarthritis

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Title: Detection of proteoglycan loss from articular cartilage using Brillouin microscopy, with applications to osteoarthritis
Authors: Wu, P-J
Masouleh, MI
Paterson, C
Dini, D
Török, P
Overby, DR
Kabakova, IV
Item Type: Journal Article
Abstract: The degeneration of articular cartilage (AC) occurs in osteoarthritis (OA), which is a leading cause of pain and disability in middle-aged and older people. The early disease-related changes in cartilage extra-cellular matrix (ECM) start with depletion of proteoglycan (PG), leading to an increase in tissue hydration and permeability. These early compositional changes are small (<10%) and hence difficult to register with conventional non-invasive imaging technologies (magnetic resonance and ultrasound imaging). Here we apply Brillouin microscopy for detecting changes in the mechanical properties and composition of porcine AC. OA-like degradation is mimicked by enzymatic tissue digestion, and we compare Brillouin microscopy measurements against histological staining of PG depletion over varying digestion times and enzyme concentrations. The non-destructive nature of Brillouin imaging technology opens new avenues for creating minimally invasive arthroscopic devices for OA diagnostics and therapeutic monitoring.
Issue Date: 1-May-2019
Date of Acceptance: 22-Feb-2019
URI: http://hdl.handle.net/10044/1/70296
DOI: https://dx.doi.org/10.1364/BOE.10.002457
ISSN: 2156-7085
Publisher: Optical Society of America
Start Page: 2457
End Page: 2466
Journal / Book Title: Biomedical Optics Express
Volume: 10
Issue: 5
Copyright Statement: © 2019 Optical Society of America under the terms of theOSA Open Access Publishing Agreement (https://www.osapublishing.org/library/license_v1.cfm#VOR-OA)
Sponsor/Funder: Engineering & Physical Science Research Council (EPSRC)
Funder's Grant Number: EP/N025954/1
Keywords: Science & Technology
Life Sciences & Biomedicine
Physical Sciences
Biochemical Research Methods
Optics
Radiology, Nuclear Medicine & Medical Imaging
Biochemistry & Molecular Biology
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Publication Status: Published
Appears in Collections:Mechanical Engineering
Physics
Bioengineering
Photonics
Faculty of Natural Sciences