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In vivo fluorescence lifetime optical projection tomography

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Title: In vivo fluorescence lifetime optical projection tomography
Authors: McGinty, J
Taylor, HB
Chen, L
Bugeon, L
Lamb, JR
Dallman, MJ
French, PMW
Item Type: Journal Article
Abstract: We demonstrate the application of fluorescence lifetime optical projection tomography (FLIM-OPT) to in vivo imaging of lysC:GFP transgenic zebrafish embryos (Danio rerio). This method has been applied to unambiguously distinguish between the fluorescent protein (GFP) signal in myeloid cells from background autofluorescence based on the fluorescence lifetime. The combination of FLIM, an inherently ratiometric method, in conjunction with OPT results in a quantitative 3-D tomographic technique that could be used as a robust method for in vivo biological and pharmaceutical research, for example as a readout of Förster resonance energy transfer based interactions.
Issue Date: 1-May-2011
Date of Acceptance: 21-Apr-2011
URI: http://hdl.handle.net/10044/1/69706
DOI: https://dx.doi.org/10.1364/BOE.2.001340
ISSN: 2156-7085
Publisher: Optical Society of America
Start Page: 1340
End Page: 1350
Journal / Book Title: Biomedical Optics Express
Volume: 2
Issue: 5
Copyright Statement: © 2011 Optical Society of America.
Keywords: Science & Technology
Life Sciences & Biomedicine
Physical Sciences
Biochemical Research Methods
Optics
Radiology, Nuclear Medicine & Medical Imaging
Biochemistry & Molecular Biology
PLANE ILLUMINATION MICROSCOPY
RESONANCE ENERGY-TRANSFER
IMAGING MICROSCOPY
DRUG DISCOVERY
ZEBRAFISH
MODEL
FRET
RESOLUTION
CELL
RECONSTRUCTION
(170.3650) Lifetime-based sensing
(170.6900) Three-dimensional microscopy
(170.6920) Time-resolved imaging
(170.6960) Tomography
Publication Status: Published
Online Publication Date: 2011-04-26
Appears in Collections:Physics
Photonics