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NaCl triggers the CRP-dependent increase of cAMP in Mycobacterium tuberculosis

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Title: NaCl triggers the CRP-dependent increase of cAMP in Mycobacterium tuberculosis
Authors: Rebollo-Ramirez, S
Larrouy-Maumus, G
Item Type: Journal Article
Abstract: The second messenger 3′,5′-cyclic adenosine monophosphate (3′,5′-cAMP) has been shown to be involved in the regulation of many biological processes ranging from carbon catabolite repression in bacteria to cell signalling in eukaryotes. In mycobacteria, the role of cAMP and the mechanisms utilized by the bacterium to adapt to and resist immune and pharmacological sterilization remain poorly understood. Among the stresses encountered by bacteria, ionic and non-ionic osmotic stresses are among the best studied. However, in mycobacteria, the link between ionic osmotic stress, particularly sodium chloride, and cAMP has been relatively unexplored. Using a targeted metabolic analysis combined with stable isotope tracing, we show that the pathogenic Mycobacterium tuberculosis but not the opportunistic pathogen Mycobacterium marinum nor the non-pathogenic Mycobacterium smegmatis responds to NaCl stress via an increase in intracellular cAMP levels. We further showed that this increase in cAMP is dependent on the cAMP receptor protein and in part on the threonine/serine kinase PnkD, which has previously been associated with the NaCl stress response in mycobacteria.
Issue Date: 31-May-2019
Date of Acceptance: 26-Mar-2019
URI: http://hdl.handle.net/10044/1/69660
DOI: https://dx.doi.org/10.1016/j.tube.2019.03.009
ISSN: 1472-9792
Publisher: Elsevier BV
Start Page: 8
End Page: 16
Journal / Book Title: Tuberculosis
Volume: 116
Copyright Statement: © 2019 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY license (http://creativecommons.org/licenses/BY/4.0/).
Sponsor/Funder: Wellcome Trust
Engineering & Physical Science Research Council (EPSRC)
Engineering & Physical Science Research Council (EPSRC)
Funder's Grant Number: 105603/Z/14/Z
EP/M027007/1
EP/M027007/1
Keywords: Microbiology
11 Medical and Health Sciences
Publication Status: Published
Online Publication Date: 2019-03-30
Appears in Collections:Faculty of Natural Sciences



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