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Chicken embryonic-stem cells are permissive to poxvirus recombinant vaccine vectors

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Title: Chicken embryonic-stem cells are permissive to poxvirus recombinant vaccine vectors
Authors: Giotis, E
Montillet, G
Pain, B
Skinner, M
Item Type: Journal Article
Abstract: The discovery of mammalian pluripotent embryonic stem cells (ESC) has revolutionised cell research and regenerative medicine. More recently discovered chicken ESC (cESC), though less intensively studied, are increasingly popular as vaccine substrates due to a dearth of avian cell lines. Information on the comparative performance of cESC with common vaccine viruses is limited. Using RNA-sequencing, we compared cESC transcriptional programmes elicited by stimulation with chicken type I interferon or infection with vaccine viruses routinely propagated in primary chicken embryo fibroblasts (CEF). We used poxviruses (fowlpox virus (FWPV) FP9, canarypox virus (CNPV), and modified vaccinia virus Ankara (MVA)) and a birnavirus (infectious bursal disease virus (IBDV) PBG98). Interferon-stimulated genes (ISGs) were induced in cESC to levels comparable to those in CEF and immortalised chicken fibroblast DF-1 cells. cESC are permissive (with distinct host transcriptional responses) to MVA, FP9, and CNPV but, surprisingly, not to PBG98. MVA, CNPV, and FP9 suppressed innate immune responses, while PBG98 induced a subset of ISGs. Dysregulation of signalling pathways (i.e., NFκB, TRAF) was observed, which might affect immune responses and viral replication. In conclusion, we show that cESC are an attractive alternative substrate to study and propagate poxvirus recombinant vaccine vectors.
Issue Date: 20-Mar-2019
Date of Acceptance: 15-Mar-2019
URI: http://hdl.handle.net/10044/1/69511
DOI: https://dx.doi.org/10.3390/genes10030237
ISSN: 2073-4425
Publisher: MDPI
Journal / Book Title: Genes
Volume: 10
Issue: 3
Copyright Statement: © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Sponsor/Funder: Biotechnology and Biological Sciences Research Council (BBSRC)
Funder's Grant Number: BB/K002465/1
Keywords: RNA-sequencing
embryonic stem cells
recombinant vaccine viruses
Publication Status: Published
Article Number: 237
Online Publication Date: 2019-03-20
Appears in Collections:Department of Medicine (up to 2019)