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Essential role of inverted repeat in Epstein–Barr virus IR-1 in B cell transformation; geographical variation of the viral genome

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Title: Essential role of inverted repeat in Epstein–Barr virus IR-1 in B cell transformation; geographical variation of the viral genome
Authors: Bridges, R
Correia, S
Wegner, F
Venturini, C
Palser, A
White, R
Kellam, P
Breuer, J
Farrell, P
Item Type: Journal Article
Abstract: Many regions of the Epstein‐Barr virus (EBV) genome, repeated and unique sequences, contribute to the geographic variation observed between strains. Here we use a large alignment of curated EBV genome sequences to identify major sites of variation in the genome of type 1 EBV strains; the CAO deletion in latent membrane protein 1 (LMP1) is the most frequent major indel present in the unique regions of EBV strains from various parts of the world. Principal component analysis was used to identify patterns of sequence variation and nucleotide positions in the sequences which can distinguish EBV from some different geographic regions. Viral genome sequence variation also affects interpretation of genetic content; known genes, origins of replication and gene expression control regions explain most of the viral genome but there are still a few sections of unknown function. One of these EBV genome regions contains a large inverted repeat sequence (invR) within the IR‐1 major internal repeat array. We deleted this invR sequence and showed that this abolished the ability of the virus to transform human B cells into lymphoblastoid cell lines.
Issue Date: May-2019
Date of Acceptance: 10-Jan-2019
URI: http://hdl.handle.net/10044/1/67185
DOI: https://doi.org/10.1098/rstb.2018.0299
ISSN: 0962-8436
Publisher: Royal Society, The
Journal / Book Title: Philosophical Transactions B: Biological Sciences
Volume: 374
Issue: 1773
Copyright Statement: © 2019 The Authors. Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/ , which permits unrestricted use, provided the original author and source are credited.
Sponsor/Funder: Medical Research Council
Research Councils UK
Funder's Grant Number: MR/N010388/1
Keywords: Science & Technology
Life Sciences & Biomedicine
Life Sciences & Biomedicine - Other Topics
Epstein-Barr virus
strain variation
cell transformation
Epstein–Barr virus
cell transformation
strain variation
06 Biological Sciences
11 Medical and Health Sciences
Evolutionary Biology
Publication Status: Published
Article Number: 20180299
Online Publication Date: 2019-04-08
Appears in Collections:Department of Infectious Diseases