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Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines

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Title: Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines
Authors: Thery, C
Witwer, KW
Aikawa, E
Jose Alcaraz, M
Anderson, JD
Andriantsitohaina, R
Antoniou, A
Arab, T
Archer, F
Atkin-Smith, GK
Ayre, DC
Bach, J-M
Bachurski, D
Baharvand, H
Balaj, L
Baldacchino, S
Bauer, NN
Baxter, AA
Bebawy, M
Beckham, C
Zavec, AB
Benmoussa, A
Berardi, AC
Bergese, P
Bielska, E
Blenkiron, C
Bobis-Wozowicz, S
Boilard, E
Boireau, W
Bongiovanni, A
Borras, FE
Bosch, S
Boulanger, CM
Breakefield, X
Breglio, AM
Brennan, MA
Brigstock, DR
Brisson, A
Broekman, MLD
Bromberg, JF
Bryl-Gorecka, P
Buch, S
Buck, AH
Burger, D
Busatto, S
Buschmann, D
Bussolati, B
Buzas, E
Byrd, JB
Camussi, G
Carter, DRF
Caruso, S
Chamley, LW
Chang, Y-T
Chen, C
Chen, S
Cheng, L
Chin, AR
Clayton, A
Clerici, SP
Cocks, A
Cocucci, E
Coffey, RJ
Cordeiro-da-Silva, A
Couch, Y
Coumans, FAW
Coyle, B
Crescitelli, R
Criado, MF
D'Souza-Schorey, C
Das, S
Chaudhuri, AD
De Candia, P
De Santana Junior, EF
De Wever, O
Del Portillo, HA
Demaret, T
Deville, S
Devitt, A
Dhondt, B
Di Vizio, D
Dieterich, LC
Dolo, V
Dominguez Rubio, AP
Dominici, M
Dourado, MR
Driedonks, TAP
Duarte, F
Duncan, HM
Eichenberger, RM
Ekstrom, K
Andaloussi, SEL
Elie-Caille, C
Erdbrugger, U
Falcon-Perez, JM
Fatima, F
Fish, JE
Flores-Bellver, M
Forsonits, A
Frelet-Barrand, A
Fricke, F
Fuhrmann, G
Gabrielsson, S
Gamez-Valero, A
Gardiner, C
Gaertner, K
Gaudin, R
Gho, YS
Giebel, B
Gilbert, C
Gimona, M
Giusti, I
Goberdhan, DC
Goergens, A
Gorski, SM
Greening, DW
Gross, JC
Gualerzi, A
Gupta, GN
Gustafson, D
Handberg, A
Haraszti, RA
Harrison, P
Hegyesi, H
Hendrix, A
Hill, AF
Hochberg, FH
Hoffmann, KF
Holder, B
Holthofer, H
Hosseinkhani, B
Hu, G
Huang, Y
Huber, V
Hunt, S
Ibrahim, AG-E
Ikezu, T
Inal, JM
Isin, M
Ivanova, A
Jackson, HK
Jacobsen, S
Jay, SM
Jayachandran, M
Jenster, G
Jiang, L
Johnson, SM
Jones, JC
Jong, A
Jovanovic-Talisman, T
Jung, S
Kalluri, R
Kano, S-I
Kaur, S
Kawamura, Y
Keller, ET
Khamari, D
Khomyakova, E
Khvorova, A
Kierulf, P
Kim, KP
Kislinger, T
Klingeborn, M
Klinke, DJ
Kornek, M
Kosanovic, MM
Kovacs, AF
Kraemer-Albers, E-M
Krasemann, S
Krause, M
Kurochkin, I
Kusuma, GD
Kuypers, S
Laitinen, S
Langevin, SM
Languino, LR
Lannigan, J
Lasser, C
Laurent, LC
Lavieu, G
Lazaro-Ibanez, E
Le Lay, S
Lee, M-S
Lee, YXF
Lemos, DS
Lenassi, M
Leszczynska, A
Li, ITS
Liao, K
Libregts, SF
Ligeti, E
Lim, R
Lim, SK
Line, A
Linnemannstoens, K
Llorente, A
Lombard, CA
Lorenowicz, MJ
Lorincz, AM
Lotvall, J
Lovett, J
Lowry, MC
Loyer, X
Lu, Q
Lukomska, B
Lunavat, TR
Maas, SLN
Malhi, H
Marcilla, A
Mariani, J
Mariscal, J
Martens-Uzunova, ES
Martin-Jaular, L
Martinez, MC
Martins, VR
Mathieu, M
Mathivanan, S
Maugeri, M
McGinnis, LK
McVey, MJ
Meckes, DG
Meehan, KL
Mertens, I
Minciacchi, VR
Moller, A
Jorgensen, MM
Morales-Kastresana, A
Morhayim, J
Mullier, F
Muraca, M
Musante, L
Mussack, V
Muth, DC
Myburgh, KH
Najrana, T
Nawaz, M
Nazarenko, I
Nejsum, P
Neri, C
Neri, T
Nieuwland, R
Nimrichter, L
Nolan, JP
Nolte-'t Hoen, ENM
Hooten, NN
O'Driscoll, L
O'Grady, T
O'Loghlen, A
Ochiya, T
Olivier, M
Ortiz, A
Ortiz, LA
Osteikoetxea, X
Ostegaard, O
Ostrowski, M
Park, J
Pegtel, DM
Peinado, H
Perut, F
Pfaffl, MW
Phinney, DG
Pieters, BCH
Pink, RC
Pisetsky, DS
Von Strandmann, EP
Polakovicova, I
Poon, IKH
Powell, BH
Prada, I
Pulliam, L
Quesenberry, P
Radeghieri, A
Raffai, RL
Raimondo, S
Rak, J
Ramirez, M
Raposo, G
Rayyan, MS
Regev-Rudzki, N
Ricklefs, FL
Robbins, PD
Roberts, DD
Rodrigues, SC
Rohde, E
Rome, S
Rouschop, KMA
Rughetti, A
Russell, AE
Saa, P
Sahoo, S
Salas-Huenuleo, E
Sanchez, C
Saugstad, JA
Saul, MJ
Schiffelers, RM
Schneider, R
Schoyen, TH
Scott, A
Shahaj, E
Sharma, S
Shatnyeva, O
Shekari, F
Shelke, GV
Shetty, AK
Shiba, K
Siljander, PR-M
Silva, AM
Skowronek, A
Snyder, OL
Soares, RP
Sodar, BW
Soekmadji, C
Sotillo, J
Stahl, PD
Stoorvogel, W
Stott, SL
Strasser, EF
Swift, S
Tahara, H
Tewari, M
Timms, K
Tiwari, S
Tixeira, R
Tkach, M
Toh, WS
Tomasini, R
Torrecilhas, AC
Pablo Tosar, J
Toxavidis, V
Urbanelli, L
Vader, P
Van Balkom, BWM
Van der Grein, SG
Van Deun, J
Van Herwijnen, MJC
Van Keuren-Jensen, K
Van Niel, G
Van Royen, ME
Van Wijnen, AJ
Helena Vasconcelos, M
Vechetti, IJ
Veit, TD
Vella, LJ
Velot, E
Verweij, FJ
Vestad, B
Vinas, JL
Visnovitz, T
Vukman, KV
Wahlgren, J
Watson, DC
Wauben, MHM
Weaver, A
Webber, JP
Weber, V
Wehman, AM
Weiss, DJ
Welsh, JA
Wendt, S
Wheelock, AM
Wiener, Z
Witte, L
Wolfram, J
Xagorari, A
Xander, P
Xu, J
Yan, X
Yanez-Mo, M
Yin, H
Yuana, Y
Zappulli, V
Zarubova, J
Zekas, V
Zhang, J-Y
Zhao, Z
Zheng, L
Zheutlin, AR
Zickler, AM
Zimmermann, P
Zivkovic, AM
Zocco, D
Zuba-Surma, EK
Item Type: Journal Article
Abstract: The last decade has seen a sharp increase in the number of scientific publications describing physiological and pathological functions of extracellular vesicles (EVs), a collective term covering various subtypes of cell-released, membranous structures, called exosomes, microvesicles, microparticles, ectosomes, oncosomes, apoptotic bodies, and many other names. However, specific issues arise when working with these entities, whose size and amount often make them difficult to obtain as relatively pure preparations, and to characterize properly. The International Society for Extracellular Vesicles (ISEV) proposed Minimal Information for Studies of Extracellular Vesicles (“MISEV”) guidelines for the field in 2014. We now update these “MISEV2014” guidelines based on evolution of the collective knowledge in the last four years. An important point to consider is that ascribing a specific function to EVs in general, or to subtypes of EVs, requires reporting of specific information beyond mere description of function in a crude, potentially contaminated, and heterogeneous preparation. For example, claims that exosomes are endowed with exquisite and specific activities remain difficult to support experimentally, given our still limited knowledge of their specific molecular machineries of biogenesis and release, as compared with other biophysically similar EVs. The MISEV2018 guidelines include tables and outlines of suggested protocols and steps to follow to document specific EV-associated functional activities. Finally, a checklist is provided with summaries of key points.
Issue Date: 1-Dec-2018
Date of Acceptance: 25-Sep-2018
URI: http://hdl.handle.net/10044/1/66229
DOI: https://dx.doi.org/10.1080/20013078.2018.1535750
ISSN: 2001-3078
Publisher: Co-Action Publishing
Journal / Book Title: Journal of Extracellular Vesicles
Volume: 7
Issue: 1
Copyright Statement: © 2018 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group on behalf of The International Society for Extracellular Vesicles. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/ ), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Keywords: Science & Technology
Life Sciences & Biomedicine
Cell Biology
extracellular vesicles
exosomes
ectosomes
microvesicles
minimal information requirements
guidelines
standardization
microparticles
rigor
reproducibility
CELL-DERIVED MICROPARTICLES
SURFACE-PLASMON RESONANCE
SIZE-EXCLUSION CHROMATOGRAPHY
FIELD-FLOW FRACTIONATION
HUMAN URINARY EXOSOMES
PROSTATE-CANCER
MEMBRANE-VESICLES
FETAL BOVINE
CIRCULATING MICROPARTICLES
PREANALYTICAL PARAMETERS
Publication Status: Published
Open Access location: https://www.tandfonline.com/doi/full/10.1080/20013078.2018.1535750
Article Number: 1535750
Online Publication Date: 2018-11-23
Appears in Collections:Department of Surgery and Cancer