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A bioinspired 3D micro-structure for graphene-based bacteria sensing

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Title: A bioinspired 3D micro-structure for graphene-based bacteria sensing
Authors: Li, B
Tan, H
Anastasova-Ivanova, S
Power, M
Seichepine, F
Yang, G-Z
Item Type: Journal Article
Abstract: Nature is a great source of inspiration for the development of solutions for biomedical problems. We present a novel biosensor design utilizing two-photon polymerisation and graphene to fabricate an enhanced biosensing platform for the detection of motile bacteria. A cage comprising venous valve-inspired directional micro-structure is fabricated around graphene-based sensing electronics. The asymmetric 3D micro-structure promotes motile cells to swim from outside the cage towards the inner-most chamber, resulting in concentrated bacteria surrounding the central sensing region, thus enhancing the sensing signal. The concentrating effect is proved across a range of cell cultures - from 101 CFU/ml to 109 CFU/ml. Fluorescence analysis shows a 3.38–3.5 times enhanced signal. pH sensor presents a 2.14–3.08 times enhancement via the detection of cellar metabolite. Electrical measurements demonstrate an 8.8–26.7 times enhanced current. The proposed platform provides a new way of leveraging bio-inspired 3D printing and 2D materials for the development of sensing devices for biomedical applications.
Issue Date: 1-Jan-2019
Date of Acceptance: 26-Sep-2018
URI: http://hdl.handle.net/10044/1/65140
DOI: 10.1016/j.bios.2018.09.087
ISSN: 0956-5663
Publisher: Elsevier
Start Page: 77
End Page: 84
Journal / Book Title: Biosensors and Bioelectronics
Volume: 123
Issue: 1
Copyright Statement: © 2018 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/BY/4.0/).
Sponsor/Funder: Engineering & Physical Science Research Council (EPSRC)
Engineering & Physical Science Research Council (EPSRC)
Engineering & Physical Science Research Council (EPSRC)
Engineering & Physical Science Research Council (EPSRC)
Funder's Grant Number: EP/L014149/1
EP/P012779/1
EP/N027132/1
EP/J021199/1
Keywords: Science & Technology
Life Sciences & Biomedicine
Physical Sciences
Biophysics
Biotechnology & Applied Microbiology
Chemistry, Analytical
Electrochemistry
Nanoscience & Nanotechnology
Chemistry
Science & Technology - Other Topics
Graphene
Label-free bacteria sensing
3D printing
Microfabrication
Bio-inspired device
ESCHERICHIA-COLI
BIOSENSORS
FABRICATION
TRANSISTOR
VERSATILE
CELLS
OXIDE
ION
3D printing
Bio-inspired device
Graphene
Label-free bacteria sensing
Microfabrication
Bacteria
Bacterial Physiological Phenomena
Biosensing Techniques
Cell Movement
Electrochemical Techniques
Graphite
Bacteria
Graphite
Biosensing Techniques
Cell Movement
Bacterial Physiological Phenomena
Electrochemical Techniques
0301 Analytical Chemistry
0903 Biomedical Engineering
1007 Nanotechnology
Bioinformatics
Publication Status: Published
Online Publication Date: 2018-09-27
Appears in Collections:Faculty of Medicine
Institute of Global Health Innovation
Department of Brain Sciences
Faculty of Engineering