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A bioinspired 3D micro-structure for graphene-based bacteria sensing
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1-s2.0-S095656631830784X-main.pdf | Published version | 2.45 MB | Adobe PDF | View/Open |
Title: | A bioinspired 3D micro-structure for graphene-based bacteria sensing |
Authors: | Li, B Tan, H Anastasova-Ivanova, S Power, M Seichepine, F Yang, G-Z |
Item Type: | Journal Article |
Abstract: | Nature is a great source of inspiration for the development of solutions for biomedical problems. We present a novel biosensor design utilizing two-photon polymerisation and graphene to fabricate an enhanced biosensing platform for the detection of motile bacteria. A cage comprising venous valve-inspired directional micro-structure is fabricated around graphene-based sensing electronics. The asymmetric 3D micro-structure promotes motile cells to swim from outside the cage towards the inner-most chamber, resulting in concentrated bacteria surrounding the central sensing region, thus enhancing the sensing signal. The concentrating effect is proved across a range of cell cultures - from 101 CFU/ml to 109 CFU/ml. Fluorescence analysis shows a 3.38–3.5 times enhanced signal. pH sensor presents a 2.14–3.08 times enhancement via the detection of cellar metabolite. Electrical measurements demonstrate an 8.8–26.7 times enhanced current. The proposed platform provides a new way of leveraging bio-inspired 3D printing and 2D materials for the development of sensing devices for biomedical applications. |
Issue Date: | 1-Jan-2019 |
Date of Acceptance: | 26-Sep-2018 |
URI: | http://hdl.handle.net/10044/1/65140 |
DOI: | 10.1016/j.bios.2018.09.087 |
ISSN: | 0956-5663 |
Publisher: | Elsevier |
Start Page: | 77 |
End Page: | 84 |
Journal / Book Title: | Biosensors and Bioelectronics |
Volume: | 123 |
Issue: | 1 |
Copyright Statement: | © 2018 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/BY/4.0/). |
Sponsor/Funder: | Engineering & Physical Science Research Council (EPSRC) Engineering & Physical Science Research Council (EPSRC) Engineering & Physical Science Research Council (EPSRC) Engineering & Physical Science Research Council (EPSRC) |
Funder's Grant Number: | EP/L014149/1 EP/P012779/1 EP/N027132/1 EP/J021199/1 |
Keywords: | Science & Technology Life Sciences & Biomedicine Physical Sciences Biophysics Biotechnology & Applied Microbiology Chemistry, Analytical Electrochemistry Nanoscience & Nanotechnology Chemistry Science & Technology - Other Topics Graphene Label-free bacteria sensing 3D printing Microfabrication Bio-inspired device ESCHERICHIA-COLI BIOSENSORS FABRICATION TRANSISTOR VERSATILE CELLS OXIDE ION 3D printing Bio-inspired device Graphene Label-free bacteria sensing Microfabrication Bacteria Bacterial Physiological Phenomena Biosensing Techniques Cell Movement Electrochemical Techniques Graphite Bacteria Graphite Biosensing Techniques Cell Movement Bacterial Physiological Phenomena Electrochemical Techniques 0301 Analytical Chemistry 0903 Biomedical Engineering 1007 Nanotechnology Bioinformatics |
Publication Status: | Published |
Online Publication Date: | 2018-09-27 |
Appears in Collections: | Faculty of Medicine Institute of Global Health Innovation Department of Brain Sciences Faculty of Engineering |