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Nanoscale tweezers for single cell biopsies

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Title: Nanoscale tweezers for single cell biopsies
Authors: Paulose Nadappuram, B
Cadinu, P
Barik, A
Ainscough, A
Devine, M
Kang, M
Gonzalez-Garcia, J
Kittler, J
Willison, K
Vilar Compte, R
Actis, P
Wojciak-Stothard, B
Oh, S-H
Ivanov, A
Edel, JB
Item Type: Journal Article
Abstract: Much of the functionality of multi - cellular systems arises from the spatial organisation and dynamic behaviours within and between cells. Current single - cell genomic methods only provide a transcriptional “snapshot” of individual cells. The real - time analy sis and perturbation of living cells would generate a step - change in single - cell analysis. Here we describe minimally invasive nanotweezers that can be spatially controlled to extract samples from living cells with single - molecule precision. They consist o f two closely spaced electrodes with gaps as small as 10 - 20 nm, which can be used for the dielectrophoretic trapping of DNA and proteins . Aside from trapping single molecules, we also extract nucleic acids for gene expression analysis from living cells, wi thout affecting their viability. Finally, we report on the trapping, and extraction of a single mitochondrion. This work bridges the gap between single - molecule/organelle manipulation and cell biology and can ultimately enable a better understanding of liv ing cells.
Issue Date: 1-Jan-2019
Date of Acceptance: 19-Oct-2018
URI: http://hdl.handle.net/10044/1/64738
DOI: 10.1038/s41565-018-0315-8
ISSN: 1748-3387
Publisher: Nature Research
Start Page: 80
End Page: 88
Journal / Book Title: Nature Nanotechnology
Volume: 14
Issue: 1
Copyright Statement: © 2018 Springer Nature Publishing AG
Sponsor/Funder: Commission of the European Communities
Engineering & Physical Science Research Council (EPSRC)
Commission of the European Communities
Biotechnology and Biological Sciences Research Council (BBSRC)
British Heart Foundation
Imperial College London
Funder's Grant Number: 279818
EP/P011985/1
724300
BB/R022429/1
FS/17/64/33476
Keywords: Science & Technology
Technology
Nanoscience & Nanotechnology
Materials Science, Multidisciplinary
Science & Technology - Other Topics
Materials Science
COPY-NUMBER VARIATION
CHEMICAL-ANALYSIS
LIVING CELLS
MANIPULATION
MOLECULE
SEPARATION
REVEALS
SYSTEM
AFM
Animals
Axons
Biopsy
Cell Line, Tumor
Cell Nucleus
DNA
Electricity
Electrodes
Fluorescence
Humans
Mice
Mitochondria
Nanotechnology
Optical Tweezers
RNA, Messenger
Single-Cell Analysis
Solutions
Axons
Cell Line, Tumor
Cell Nucleus
Mitochondria
Animals
Humans
Mice
DNA
RNA, Messenger
Solutions
Biopsy
Electrodes
Electricity
Nanotechnology
Fluorescence
Optical Tweezers
Single-Cell Analysis
Nanoscience & Nanotechnology
Publication Status: Published
Online Publication Date: 2018-12-03
Appears in Collections:Chemistry
Biological and Biophysical Chemistry
National Heart and Lung Institute
Faculty of Medicine
Faculty of Natural Sciences