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Characterization of NAD(P)H and FAD autofluorescence signatures in a Langendorff isolated-perfused rat heart model

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Title: Characterization of NAD(P)H and FAD autofluorescence signatures in a Langendorff isolated-perfused rat heart model
Authors: Lagarto, J
Dyer, B
Talbot, C
Peters, N
French, P
Lyon, A
Dunsby, C
Item Type: Journal Article
Abstract: Autofluorescence spectroscopy is a promising label-free approach to characterize biological samples with demonstrated potential to report structural and biochemical alterations in tissues in a number of clinical applications. We report a characterization of the ex vivo autofluorescence fingerprint of cardiac tissue, exploiting a Langendorff-perfused isolated rat heart model to induce physiological insults to the heart, with a view to understanding how metabolic alterations affect the autofluorescence signals. Changes in the autofluorescence intensity and lifetime signatures associated with reduced nicotinamide adenine dinucleotide (phosphate) (NAD(P)H) and flavin adenine dinucleotide (FAD) were characterized during oxygen- or glucose-depletion protocols. Results suggest that both NAD(P)H and FAD autofluorescence intensity and lifetime parameters are sensitive to changes in the metabolic state of the heart owing to oxygen deprivation. We also observed changes in NAD(P)H fluorescence intensity and FAD lifetime parameter on reperfusion of oxygen, which might provide information on reperfusion injury, and permanent tissue damage or changes to the tissue during recovery from oxygen deprivation. We found that changes in the autofluorescence signature following glucose-depletion are, in general, less pronounced, and most clearly visible in NAD(P)H related parameters. Overall, the results reported in this investigation can serve as baseline for future investigations of cardiac tissue involving autofluorescence measurements.
Issue Date: 21-Sep-2018
Date of Acceptance: 14-Aug-2018
URI: http://hdl.handle.net/10044/1/63497
DOI: https://dx.doi.org/10.1364/BOE.9.004961
ISSN: 2156-7085
Publisher: Optical Society of America
Start Page: 4978
End Page: 4978
Journal / Book Title: Biomedical Optics Express
Volume: 9
Issue: 10
Copyright Statement: © 2018 The Author(s). Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article’s title, journal citation, and DOI. (https://creativecommons.org/licenses/by/4.0/)
Sponsor/Funder: Engineering & Physical Science Research Council (EPSRC)
British Heart Foundation
Funder's Grant Number: EP/I02770X/1
Publication Status: Published
Appears in Collections:Physics
National Heart and Lung Institute
Faculty of Natural Sciences