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Full-genome deep sequencing and phylogenetic analysis of novel human betacoronavirus.
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Full-genome deep sequencing and phylogenetic analysis of novel human betacoronavirus.pdf | Published version | 1.08 MB | Adobe PDF | View/Open |
Title: | Full-genome deep sequencing and phylogenetic analysis of novel human betacoronavirus. |
Authors: | Cotten, M Lam, TT Watson, SJ Palser, AL Petrova, V Grant, P Pybus, OG Rambaut, A Guan, Y Pillay, D Kellam, P Nastouli, E |
Item Type: | Journal Article |
Abstract: | A novel betacoronavirus associated with lethal respiratory and renal complications was recently identified in patients from several countries in the Middle East. We report the deep genome sequencing of the virus directly from a patient's sputum sample. Our high-throughput sequencing yielded a substantial depth of genome sequence assembly and showed the minority viral variants in the specimen. Detailed phylogenetic analysis of the virus genome (England/Qatar/2012) revealed its close relationship to European bat coronaviruses circulating among the bat species of the Vespertilionidae family. Molecular clock analysis showed that the 2 human infections of this betacoronavirus in June 2012 (EMC/2012) and September 2012 (England/Qatar/2012) share a common virus ancestor most likely considerably before early 2012, suggesting the human diversity is the result of multiple zoonotic events. |
Issue Date: | 1-May-2013 |
Date of Acceptance: | 1-May-2013 |
URI: | http://hdl.handle.net/10044/1/62235 |
DOI: | https://dx.doi.org/10.3201/eid1905.130057 |
ISSN: | 1080-6040 |
Publisher: | U.S. National Center for Infectious Diseases |
Start Page: | 736 |
End Page: | 742B |
Journal / Book Title: | Emerging Infectious Diseases |
Volume: | 19 |
Issue: | 5 |
Copyright Statement: | © 2013 The Author(s). This article is open access under a Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). |
Keywords: | computer-aided design coronavirus infections disease transmission evolution high-throughput nucleotide sequencing infectious molecular respiratory tract infections viruses zoonoses Animals Base Sequence Chiroptera Coronavirus Coronavirus Infections DNA Primers DNA, Complementary Genome, Viral High-Throughput Nucleotide Sequencing Humans Jordan Molecular Sequence Data Phylogeny Qatar RNA, Viral Reverse Transcriptase Polymerase Chain Reaction Saudi Arabia 1108 Medical Microbiology 1117 Public Health And Health Services 1103 Clinical Sciences Microbiology |
Publication Status: | Published |
Conference Place: | United States |
Online Publication Date: | 2013-05-01 |
Appears in Collections: | Department of Medicine (up to 2019) |