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easySLM-STED: stimulated emission depletion microscopy with aberration correction, extended field of view and multiple beam scanning

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Title: easySLM-STED: stimulated emission depletion microscopy with aberration correction, extended field of view and multiple beam scanning
Authors: Gorlitz, F
Guldbrand, S
Runcorn, T
Murray, R
Jaso-Tamame, A
Sinclair, H
Martinez-Perez, E
Taylor, J
Neil, M
Dunsby, CW
French, P
Item Type: Journal Article
Abstract: We demonstrate a simplified set‐up for STED microscopy with a straightforward alignment procedure that uses a single spatial light modulator (SLM) with collinear incident excitation and depletion beams to provide phase modulation of the beam profiles and correction of optical aberrations. We show that this approach can be used to extend the field of view for STED microscopy by correcting chromatic aberration that otherwise leads to walk‐off between the focused excitation and depletion beams. We further show how this arrangement can be adapted to increase the imaging speed through multibeam excitation and depletion. Fine adjustments to the alignment can be accomplished using the SLM only, conferring the potential for automation.
Issue Date: 1-Nov-2018
Date of Acceptance: 5-Jul-2018
URI: http://hdl.handle.net/10044/1/62063
DOI: https://dx.doi.org/10.1002/jbio.201800087
ISSN: 1864-063X
Publisher: Wiley-VCH Verlag
Journal / Book Title: Journal of Biophotonics
Volume: 11
Issue: 11
Copyright Statement: © 2018 The Authors. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. https://creativecommons.org/licenses/by/4.0/
Sponsor/Funder: Medical Research Council
Medical Research Council (MRC)
Funder's Grant Number: MR/K015834/1
Keywords: Science & Technology
Life Sciences & Biomedicine
Physical Sciences
Biochemical Research Methods
Biochemistry & Molecular Biology
aberration correction
spatial light modulator
super resolved microscopy
aberration correction
spatial light modulator
super resolved microscopy
Optoelectronics & Photonics
Publication Status: Published
Article Number: e201800087
Online Publication Date: 2018-07-05
Appears in Collections:Physics
Institute of Clinical Sciences
Faculty of Natural Sciences