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S100P enhances the motility and invasion of human trophoblast cell lines

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Title: S100P enhances the motility and invasion of human trophoblast cell lines
Authors: Tabrizi, MEA
Lancaster, TL
Ismail, TM
Georgiadou, A
Ganguly, A
Mistry, JJ
Wang, K
Rudland, PS
Ahmed, S
Gross, SR
Item Type: Journal Article
Abstract: S100P has been shown to be a marker for carcinogenesis where its expression in solid tumours correlates with metastasis and a poor patient prognosis. This protein’s role in any physiological process is, however, unknown. Here we first show that S100P is expressed both in trophoblasts in vivo as well as in some corresponding cell lines in culture. We demonstrate that S100P is predominantly expressed during the early stage of placental formation with its highest expression levels occurring during the first trimester of gestation, particularly in the invading columns and anchoring villi. Using gain or loss of function studies through overexpression or knockdown of S100P expression respectively, our work shows that S100P stimulates both cell motility and cellular invasion in different trophoblastic and first trimester EVT cell lines. Interestingly, cell invasion was seen to be more dramatically affected than cell migration. Our results suggest that S100P may be acting as an important regulator of trophoblast invasion during placentation. This finding sheds new light on a hitherto uncharacterized molecular mechanism which may, in turn, lead to the identification of novel targets that may explain why significant numbers of confirmed human pregnancies suffer complications through poor placental implantation.
Issue Date: 31-Jul-2018
Date of Acceptance: 26-Jun-2018
URI: http://hdl.handle.net/10044/1/61857
DOI: https://dx.doi.org/10.1038/s41598-018-29852-2
ISSN: 2045-2322
Publisher: Nature Publishing Group
Journal / Book Title: Scientific Reports
Volume: 8
Copyright Statement: © The Author(s) 2018. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Cre- ative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not per- mitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
Keywords: Science & Technology
Multidisciplinary Sciences
Science & Technology - Other Topics
CALCIUM-BINDING PROTEIN
HUMAN EXTRAVILLOUS TROPHOBLASTS
BREAST-CANCER PATIENTS
GENE-EXPRESSION
HUMAN PLACENTA
CALBINDIN-D28K CABP28K
CA2+-BINDING PROTEIN
HUMAN ENDOMETRIUM
NONMUSCLE MYOSIN
FOCAL ADHESIONS
Publication Status: Published
Article Number: ARTN 11488
Online Publication Date: 2018-07-31
Appears in Collections:Department of Medicine (up to 2019)