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Bariatric surgery modulates circulating and cardiac metabolites

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Title: Bariatric surgery modulates circulating and cardiac metabolites
Authors: Ashrafian, H
Li, J
Spagou, K
Harling, L
Masson, P
Darzi, A
Nicholson, J
Holmes, E
Athanasiou, T
Item Type: Journal Article
Abstract: Bariatric procedures such as the Roux-en-Y gastric bypass (RYGB) operation offer profound metabolic enhancement in addition to their well-recognized weight loss effects. They are associated with significant reduction in cardiovascular disease risk and mortality, which suggests a surgical modification on cardiac metabolism. Metabolic phenotyping of the cardiac tissue and plasma postsurgery may give insight into cardioprotective mechanisms. The aim of the study was to compare the metabolic profiles of plasma and heart tissue extracts from RYGB- and sham-operated Wistar rats to identify the systemic and cardiac signature of metabolic surgery. A total of 27 male Wistar rats were housed individually for a week and subsequently underwent RYGB (n = 13) or sham (n = 14) operation. At week 8 postoperation, a total of 27 plasma samples and 16 heart tissue samples (8 RYGB; 8 Sham) were collected from animals and analyzed using 1H nuclear magnetic resonance (NMR) spectroscopy and ultra performance liquid chromatography (UPLC-MS) to characterize the global metabolite perturbation induced by RYGB operation. Plasma bile acids, phosphocholines, amino acids, energy-related metabolites, nucleosides and amine metabolites, and cardiac glycogen and amino acids were found to be altered in the RYGB operated group. Correlation networks were used to identify metabolite association. The metabolic phenotype of this bariatric surgical model inferred systematic change in both myocardial and systemic activity post surgery. The altered metabolic profile following bariatric surgery reflects an enhancement of cardiac energy metabolism through TCA cycle intermediates, cardiorenal protective activity, and biochemical caloric restriction. These surgically induced metabolic shifts identify some of the potential mechanisms that contribute toward bariatric cardioprotection through gut microbiota ecological fluxes and an enterocardiac axis to shield against metabolic syndrome of cardiac dysfunction.
Issue Date: 7-Feb-2014
Date of Acceptance: 1-Feb-2013
URI: http://hdl.handle.net/10044/1/60538
DOI: https://dx.doi.org/10.1021/pr400748f
ISSN: 1535-3893
Publisher: American Chemical Society
Start Page: 570
End Page: 580
Journal / Book Title: Journal of Proteome Research
Volume: 13
Issue: 2
Copyright Statement: © 2014 American Chemical Society. This document is the Accepted Manuscript version of a Published Work that appeared in final form in Journal of Proteome Research, after peer review and technical editing by the publisher. To access the final edited and published work see https://dx.doi.org/10.1021/pr400748f
Sponsor/Funder: National Institute for Health Research
Funder's Grant Number: NF-SI-0510-10186
Keywords: Science & Technology
Life Sciences & Biomedicine
Biochemical Research Methods
Biochemistry & Molecular Biology
bariatric surgery
cardio metabolites
metabolic profiling
metabolic surgery
NMR spectroscopy
Chromatography, Liquid
Gastric Bypass
Magnetic Resonance Spectroscopy
Rats, Wistar
Spectrometry, Mass, Electrospray Ionization
06 Biological Sciences
03 Chemical Sciences
Publication Status: Published
Online Publication Date: 2013-10-13
Appears in Collections:Department of Surgery and Cancer
Institute of Global Health Innovation