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Expanding lipidome coverage using MS/MS-aided untargeted data-independent RP-UPLC-TOF-MSE acquisition

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Title: Expanding lipidome coverage using MS/MS-aided untargeted data-independent RP-UPLC-TOF-MSE acquisition
Authors: Vorkas, PA
Item Type: Journal Article
Abstract: Lipid function and importance in disease are being rediscovered due to modern advancements in chemical analysis. RP–UPLC–TOF–MSE is now the lipidomics tool of choice and can provide the demanded specificity for detecting the great diversity of the lipidome. It can offer simplicity, rapidity, robustness and high throughputness, without the need for further optimization in current sample preparation protocols. This method can cover the major lipid categories with the ability to detect several corresponding subclasses. It can deliver adequate information for deciphering fatty chain length, unsaturation and regioisomerism. It has enabled the detection of a vast number of lipids, of which more than 250 are reported here. These lipids were detected from applications in a variety of biological matrices and species.
Issue Date: 1-Mar-2018
Date of Acceptance: 16-Jan-2018
URI: http://hdl.handle.net/10044/1/58255
DOI: https://dx.doi.org/10.4155/bio-2017-0249
ISSN: 1757-6180
Publisher: Future Science
Start Page: 307
End Page: 319
Journal / Book Title: Bioanalysis
Volume: 10
Issue: 5
Copyright Statement: © 2018 Panagiotis A Vorkas. This work is licensed under the Attribution-NonCommercial-NoDerivatives 4.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/
Keywords: Science & Technology
Life Sciences & Biomedicine
Physical Sciences
Biochemical Research Methods
Chemistry, Analytical
Biochemistry & Molecular Biology
Chemistry
data-dependent acquisition
lipidomics
MSE data-independent acquisition
untargeted analysis
MASS-SPECTROMETRY
CARDIOVASCULAR-DISEASE
ATHEROSCLEROSIS
ELUCIDATION
METABOLOME
BIOMARKER
CERAMIDE
PATHWAY
TISSUE
IONS
Publication Status: Published
Open Access location: https://www.future-science.com/doi/abs/10.4155/bio-2017-0249
Appears in Collections:Department of Surgery and Cancer