IRUS Total

Xenon protects against blast-induced traumatic brain injury in an in vitro model

File Description SizeFormat 
neu.2017.5360.pdfPublished version626.35 kBAdobe PDFView/Open
Title: Xenon protects against blast-induced traumatic brain injury in an in vitro model
Authors: Campos Pires, R
Koziakova, M
Yonis, A
Pau, A
Macdonald, W
Harris, K
Edge, C
Franks, N
Mahoney, P
Dickinson, R
Item Type: Journal Article
Abstract: The aim of this study was to evaluate the neuroprotective efficacy of the inert gas xenon as a treatment for patients with blast-induced traumatic brain injury in an in vitro laboratory model. We developed a novel blast traumatic brain injury model using C57BL/6N mouse organotypic hippocampal brain-slice cultures exposed to a single shockwave, with the resulting injury quantified using propidium iodide fluorescence. A shock tube blast generator was used to simulate open field explosive blast shockwaves, modeled by the Friedlander waveform. Exposure to blast shockwave resulted in significant (p < 0.01) injury that increased with peak-overpressure and impulse of the shockwave, and which exhibited a secondary injury development up to 72 h after trauma. Blast-induced propidium iodide fluorescence overlapped with cleaved caspase-3 immunofluorescence, indicating that shock-wave–induced cell death involves apoptosis. Xenon (50% atm) applied 1 h after blast exposure reduced injury 24 h (p < 0.01), 48 h (p < 0.05), and 72 h (p < 0.001) later, compared with untreated control injury. Xenon-treated injured slices were not significantly different from uninjured sham slices at 24 h and 72 h. We demonstrate for the first time that xenon treatment after blast traumatic brain injury reduces initial injury and prevents subsequent injury development in vitro. Our findings support the idea that xenon may be a potential first-line treatment for those with blast-induced traumatic brain injury.
Issue Date: 15-Apr-2018
Date of Acceptance: 28-Dec-2017
URI: http://hdl.handle.net/10044/1/55626
DOI: https://dx.doi.org/10.1089/neu.2017.5360
ISSN: 0897-7151
Publisher: Mary Ann Liebert
Start Page: 1037
End Page: 1044
Journal / Book Title: Journal of Neurotrauma
Volume: 35
Issue: 8
Copyright Statement: © Rita Campos-Pires et al., 2018; Published by Mary Ann Liebert, Inc. This Open Access article is distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.
Sponsor/Funder: Air Products And Chemicals Inc
Air Products And Chemicals Inc
Carburos Metalicos S.E.C.M.S.A
Carburos Metalicos S.E.C.M.S.A
Medical Research Council (MRC)
Westminster Medical School Research Trust
Royal Centre for Defence Medicine
The Royal British Legion
Medical Research Council (MRC)
Medical Research Council (MRC)
Funder's Grant Number: 084-344-P
JRC 1-/11 PHD 001
Centre for Blast Injury Studie
Keywords: TBI
blast traumatic brain injury
blast-induced neurotrauma
primary blast injury
1103 Clinical Sciences
1109 Neurosciences
Neurology & Neurosurgery
Publication Status: Published
Appears in Collections:Bioengineering
Department of Surgery and Cancer
Faculty of Natural Sciences
Faculty of Engineering