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Analysing the lattice transition of thin filaments in striated muscle

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Title: Analysing the lattice transition of thin filaments in striated muscle
Authors: Burgoyne, Thomas
Item Type: Thesis or dissertation
Abstract: Thin filaments, through interaction with thick filaments, form the contractile apparatus of striated muscle. Therefore, the length and arrangement of the thin filaments are of key importance to the function of the muscle. The thin filaments from adjacent sarcomeres are anchored at the Z-disc. In 1968 Pringle predicted that thin filament are organised in the Z-disc in a rhomboid lattice rather than a square lattice. Previous experimental evidence has been insufficient to verify Pringle’s suggestion. In the A-band the thin filaments interdigitate with the thick filaments on a hexagonal lattice, hence from the Z-disc to the A-band, there is a transition of the lattice from square to hexagonal. In this project, I have firstly used Fourier analysis and electron tomography to investigate the thin filament lattice in the Z-disc. I have used electron tomography to determine how the lattice transition occurs between the Z-disc and the A-band. Electron tomography of these samples also allowed me to determine the lengths of thin filaments, showing unequivocally that they are of variable lengths in cardiac muscle.
Issue Date: Sep-2009
Date Awarded: Mar-2010
URI: http://hdl.handle.net/10044/1/5559
DOI: https://doi.org/10.25560/5559
Supervisor: Luther, Pradeep
Sponsor/Funder: British Heart Foundation
Author: Burgoyne, Thomas
Department: National Heart and Lung Institute
Publisher: Imperial College London
Qualification Level: Doctoral
Qualification Name: Doctor of Philosophy (PhD)
Appears in Collections:National Heart and Lung Institute PhD theses



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