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Transformation asymmetry and the evolution of the bacterial accessory genome

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Title: Transformation asymmetry and the evolution of the bacterial accessory genome
Authors: Croucher, NJ
Apagyi, KJ
Fraser, C
Item Type: Journal Article
Abstract: Bacterial transformation can insert or delete genomic islands (GIs), depending on the donor and recipient genotypes, if an homologous recombination spans the GI’s integration site and includes sufficiently long flanking homologous arms. Combining mathematical models of recombination with experiments using pneumococci found GI insertion rates declined geometrically with the GI’s size. The decrease in acquisition frequency with length (1.08x10−3 bp−1) was higher than a previous estimate of the analogous rate at which core genome recombinations terminated. Although most efficient for shorter GIs, transformation-mediated deletion frequencies did not vary consistently with GI length, with removal of 10 kb GIs approximately 50% as efficient as acquisition of base substitutions. Fragments of two kilobases, typical of transformation event sizes, could drive all these deletions independent of island length. The strong asymmetry of transformation, and its capacity to efficiently remove GIs, suggests non-mobile accessory loci will decline in frequency without preservation by selection.
Issue Date: 1-Mar-2018
Date of Acceptance: 22-Nov-2017
URI: http://hdl.handle.net/10044/1/54366
DOI: https://dx.doi.org/10.1093/molbev/msx309
ISSN: 1537-1719
Publisher: Oxford University Press (OUP)
Start Page: 575
End Page: 581
Journal / Book Title: Molecular Biology and Evolution
Volume: 35
Issue: 3
Copyright Statement: © The Author(s) 2017. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
Sponsor/Funder: Medical Research Council (MRC)
Wellcome Trust
Funder's Grant Number: MR/K010174/1B
104169/Z/14/Z
Keywords: Science & Technology
Life Sciences & Biomedicine
Biochemistry & Molecular Biology
Evolutionary Biology
Genetics & Heredity
recombination
bacterial evolution
mobile elements
horizontal gene transfer
transformation
pneumococcus
STREPTOCOCCUS-PNEUMONIAE
PNEUMOCOCCAL TRANSFORMATION
GENETIC EXCHANGE
DELETIONAL BIAS
MODEL
MUTATION
ELEMENTS
ISLANDS
DNA
0604 Genetics
0603 Evolutionary Biology
0601 Biochemistry And Cell Biology
Publication Status: Published
Online Publication Date: 2017-12-01
Appears in Collections:School of Public Health