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3'-Deoxy-3'-[(18)F]Fluorothymidine uptake is related to thymidine phosphorylase expression in various experimental tumor models

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Title: 3'-Deoxy-3'-[(18)F]Fluorothymidine uptake is related to thymidine phosphorylase expression in various experimental tumor models
Authors: Schelhaas, S
Heinzmann, K
Honess, DJ
Smith, D-M
Keen, H
Heskamp, S
Witney, TH
Besret, L
Doblas, S
Griffiths, JR
Aboagye, EO
Jacobs, AH
Item Type: Journal Article
Abstract: PURPOSE: We recently reported that high thymidine phosphorylase (TP) expression is accompanied by low tumor thymidine concentration and high 3'-deoxy-3'-[(18)F]fluorothymidine ([(18)F]FLT) uptake in four untreated lung cancer xenografts. Here, we investigated whether this relationship also holds true for a broader range of tumor models. PROCEDURES: Lysates from n = 15 different tumor models originating from n = 6 institutions were tested for TP and thymidylate synthase (TS) expression using western blots. Results were correlated to [(18)F]FLT accumulation in the tumors as determined by positron emission tomography (PET) measurements in the different institutions and to previously published thymidine concentrations. RESULTS: Expression of TP correlated positively with [(18)F]FLT SUVmax (ρ = 0.549, P < 0.05). Furthermore, tumors with high TP levels possessed lower levels of thymidine (ρ = - 0.939, P < 0.001). CONCLUSIONS: In a broad range of tumors, [(18)F]FLT uptake as measured by PET is substantially influenced by TP expression and tumor thymidine concentrations. These data strengthen the role of TP as factor confounding [(18)F]FLT uptake.
Issue Date: 1-Apr-2018
Date of Acceptance: 1-Oct-2017
URI: http://hdl.handle.net/10044/1/53971
DOI: https://dx.doi.org/10.1007/s11307-017-1125-3
ISSN: 1536-1632
Publisher: Springer Verlag
Start Page: 194
End Page: 199
Journal / Book Title: Molecular Imaging and Biology
Volume: 20
Issue: 2
Copyright Statement: © World Molecular Imaging Society 2017. The final publication is available at Springer via https://link.springer.com/article/10.1007/s11307-017-1125-3
Sponsor/Funder: Commission of the European Communities
Funder's Grant Number: 115151
Keywords: Science & Technology
Life Sciences & Biomedicine
Radiology, Nuclear Medicine & Medical Imaging
[F-18] FLT
Thymidine phosphorylase
1103 Clinical Sciences
0606 Physiology
Nuclear Medicine & Medical Imaging
Publication Status: Published
Conference Place: United States
Online Publication Date: 2017-10-02
Appears in Collections:Department of Surgery and Cancer