Aqueous humor polymerase chain reaction in uveitis - utility and safety

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Title: Aqueous humor polymerase chain reaction in uveitis - utility and safety
Authors: Chronopoulos, A
Roquelaure, D
Souteyrand, G
Seebach, JD
Schutz, JS
Thumann, G
Item Type: Journal Article
Abstract: Background: To study the value and safety of aqueous humor polymerase chain reaction (PCR) analysis for Herpes simplex, varicella zoster, cytomegalovirus, Epstein-Barr virus and Toxoplasma gondii in patients with uveitis. Methods: Records of 45 consecutive patients with anterior and posterior uveitis who underwent AC paracentesis with PCR were reviewed. The main outcome measure was frequency of PCR positivity. Secondary outcomes were alteration of treatment, safety of paracentesis, and correlation of keratitic precipitates with PCR positivity, Results: The overall PCR positivity was 48.9 % (22/45). Therapy was changed because of the PCR results in 14/45 patients (37.7 %). One patient experienced a paracentesis related complication (1/45, 2.2 %) without long-term sequelae. Conclusion: Aqueous PCR altered the diagnosis and treatment in over a third of our patients and was relatively safe. Aqueous PCR should be considered for uveitis of atypical clinical appearance, recurrent severe uveitis of uncertain etiology, and therapy refractory cases.
Issue Date: 28-Oct-2016
Date of Acceptance: 20-Oct-2016
ISSN: 1471-2415
Publisher: BioMed Central
Journal / Book Title: BMC Ophthalmology
Volume: 16
Copyright Statement: © The Author(s). 2016 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (, which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver ( applies to the data made available in this article, unless otherwise stated.
Keywords: Science & Technology
Life Sciences & Biomedicine
Anterior uveitis
Posterior uveitis
Anterior chamber paracentesis
Polymerase chain reaction (PCR)
Aqueous Humor
Diagnostic Techniques, Ophthalmological
Eye Infections, Viral
Middle Aged
Polymerase Chain Reaction
Young Adult
1113 Ophthalmology And Optometry
Ophthalmology & Optometry
Publication Status: Published
Article Number: 189
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