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Towards a minimally invasive device for beta-lactam monitoring in humans

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Title: Towards a minimally invasive device for beta-lactam monitoring in humans
Authors: Rawson, TM
Sharma, S
Georgiou, P
Holmes, A
Cass, A
O'Hare, D
Item Type: Journal Article
Abstract: Antimicrobial resistance is a leading patient safety issue. There is a need to develop novel mechanisms for monitoring and subsequently improving the precision of how we use antibiotics. A surface modified microneedle array was developed for monitoring beta-lactam antibiotic levels in human interstitial fluid. The sensor was fabricated by anodically electrodepositing iridium oxide (AEIROF) onto a platinum surface on the microneedle followed by fixation of beta-lactamase enzyme within a hydrogel. Calibration of the sensor was performed to penicillin-G in buffer solution (PBS) and artificial interstitial fluid (ISF). Further calibration of a platinum disc electrode was undertaken using amoxicillin and ceftriaxone. Open-circuit potentials were performed and data analysed using the Hill equation and log(concentration [M]) plots. The microneedle sensor demonstrated high reproducibility between penicillin-G runs in PBS with mean Km (± 1SD) = 0.0044 ± 0.0013 M and mean slope function of log(concentration plots) 29 ± 1.80 mV/decade (r2 = 0.933). Response was reproducible after 28 days storage at 4 °C. In artificial ISF, the sensors response was Km (± 1SD) = 0.0077 ± 0.0187 M and a slope function of 34 ± 1.85 mv/decade (r2 = 0.995). Our results suggest that microneedle array based beta-lactam sensing may be a future application of this AEIROF based enzymatic sensor.
Issue Date: 1-Sep-2017
Date of Acceptance: 12-Jul-2017
URI: http://hdl.handle.net/10044/1/52776
DOI: 10.1016/j.elecom.2017.07.011
ISSN: 1388-2481
Publisher: Elsevier
Start Page: 1
End Page: 5
Journal / Book Title: Electrochemistry Communications
Volume: 82
Copyright Statement: © 2017 Elsevier B.V. All rights reserved. This manuscript is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/
Sponsor/Funder: Imperial College Healthcare NHS Trust- BRC Funding
Imperial College Healthcare NHS Trust- BRC Funding
National Institute for Health Research
National Institute for Health Research
Funder's Grant Number: RD011
RDA02
II-LA-0214-20008
II-LA-0214-20008
Keywords: Science & Technology
Physical Sciences
Electrochemistry
Beta-lactam antibiotic monitoring
Minimally invasive
Continuous monitoring
Antibiotic resistance
FIELD-EFFECT TRANSISTOR
CRITICALLY-ILL PATIENTS
AMINOGLYCOSIDE ANTIBIOTICS
SENSITIVE DETERMINATION
RECOGNITION ELEMENTS
POLYMERIC MEMBRANES
GRAPHENE OXIDE
POWDERED MILK
FOOD SAMPLES
PH CHANGES
Beta-lactam antibiotic monitoring
antibiotic resistance
continuous monitoring
minimally invasive
Energy
03 Chemical Sciences
09 Engineering
Publication Status: Published
Online Publication Date: 2017-07-13
Appears in Collections:Bioengineering
Chemistry
Biological and Biophysical Chemistry
Electrical and Electronic Engineering
Department of Infectious Diseases
Faculty of Medicine
Faculty of Natural Sciences
Faculty of Engineering