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Cucurbit extrafascicular phloem has strong negative impacts on aphids and is not a preferred feeding site.

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Title: Cucurbit extrafascicular phloem has strong negative impacts on aphids and is not a preferred feeding site.
Authors: Kanvil, S
Pham, J
Lopez-Cobollo, R
Selby, M
Bennett, M
Beckingham, C
Powell, G
Turnbull, C
Item Type: Journal Article
Abstract: Cucurbits have long been known to possess two types of phloem: fascicular (FP) within vascular bundles and extrafascicular phloem (EFP) surrounding vascular bundles and scattered through the cortex. Recently, their divergent composition was revealed, with FP having high sugar content consistent with conventional phloem, but EFP having much lower sugar levels and a very different proteome. However, the evolutionary advantages of possessing both FP and EFP have remained unclear. Here, we present four lines of quantitative evidence that together support the hypothesis that FP represents a typical phloem and is an attractive diet for aphids, whereas aphids avoid feeding on EFP. First, aphid stylet track endings were more abundant near the abaxial FP element of minor veins, suggesting a feeding preference for FP over EFP. Second, sugar profiles from stylet exudates were wholly consistent with FP origins, further supporting preference for FP and avoidance of EFP. Third, supplementation of EFP exudate into artificial diets confirmed an aversion to EFP in choice experiments. Finally, EFP exudate had negative effects on aphid performance. On the basis of aphids' inability to thrive on EFP, we conclude that EFP is atypical and perhaps should not be classed as a phloem system.
Issue Date: 24-Sep-2017
Date of Acceptance: 31-Jul-2017
URI: http://hdl.handle.net/10044/1/52136
DOI: https://dx.doi.org/10.1111/pce.13053
ISSN: 0140-7791
Publisher: Wiley
Start Page: 2780
End Page: 2789
Journal / Book Title: Plant, Cell and Environment
Volume: 40
Issue: 11
Copyright Statement: © 2017 John Wiley & Sons Ltd. This is the accepted version of the following article: Kanvil S, Pham J, Lopez-Cobollo R, et al. Cucurbit extrafascicular phloem has strong negative impacts on aphids and is not a preferred feeding site. Plant Cell Environ. 2017;40:2780–2789, which has been published in final form at https://dx.doi.org/10.1111/pce.13053
Sponsor/Funder: Biotechnology and Biological Sciences Research Council (BBSRC)
Funder's Grant Number: BB/I021930/1
Keywords: aphid
biotic stress
fascicular phloem
06 Biological Sciences
07 Agricultural And Veterinary Sciences
Plant Biology & Botany
Publication Status: Published
Conference Place: United States
Appears in Collections:Faculty of Natural Sciences