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High temporal resolution delayed analysis of clinical microdialysate streams

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Title: High temporal resolution delayed analysis of clinical microdialysate streams
Authors: Boutelle, MG
Gowers, SAN
Hamaoui, K
Cunnea, P
Anastasova-Ivanova, S
Curto, VF
Vadgama, P
Yang, G-Z
Papalois, V
Drakakis, EM
Weber, SG
Boutelle, MG
Item Type: Journal Article
Abstract: This paper presents the use of tubing to store clinical microdialysis samples for delayed analysis with high temporal resolution, offering an alternative to traditional discrete offline microdialysis sampling. Samples stored in this way were found to be stable for up to 72 days at −80 °C. Examples of how this methodology can be applied to glucose and lactate measurement in a wide range of in vivo monitoring experiments are presented. This paper presents a general model, which allows for an informed choice of tubing parameters for a given storage time and flow rate avoiding high back pressure, which would otherwise cause the microdialysis probe to leak, while maximising temporal resolution.
Issue Date: 16-Jan-2018
Date of Acceptance: 19-Oct-2017
URI: http://hdl.handle.net/10044/1/51964
DOI: https://dx.doi.org/10.1039/C7AN01209H
ISSN: 1364-5528
Publisher: Royal Society of Chemistry
Start Page: 715
End Page: 724
Journal / Book Title: Analyst
Volume: 143
Copyright Statement: This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.
Sponsor/Funder: Engineering & Physical Science Research Council (EPSRC)
Wellcome Trust
Ovarian Cancer Action
Funder's Grant Number: EP/H009744/1
Keywords: 0301 Analytical Chemistry
0399 Other Chemical Sciences
Analytical Chemistry
Publication Status: Published
Appears in Collections:Bioengineering
Faculty of Engineering