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Highly Sensitive Plasmonic Detection of the Pancreatic Cancer Biomarker CA 19-9

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Title: Highly Sensitive Plasmonic Detection of the Pancreatic Cancer Biomarker CA 19-9
Authors: Jawad, ZAR
Theodorou, I
Jiao, LR
Xie, F
Item Type: Journal Article
Abstract: Plasmonic gold (Au) nanotriangular arrays, functionalized with a near infrared (NIR) fuorophoreconjugated immunoassay to Carbohydrate Antigen 19-9 (CA 19-9), a pancreatic cancer biomarker, produce optically tunable substrates with two orders of magnitude fuorescence enhancement. Through nanoscale morphological control, the sensitivities of the plasmonic nanotriangular arrays are controllable, paving the way of such optical platforms for multiplexing. Here, we report a limit of detection (LOD) of 7.7×10−7 U.mL−1 for CA 19–9 by using such tunable Au nanotriangular arrays, a great improvement compared to commercially available CA 19–9 immunoassays. The linear dynamic range was from 1×10−6 U.mL−1 to 1 U.mL−1, i.e. up to six orders of magnitude. Moreover, high specifcity was demonstrated, together with successful validation in serum samples. Their superior tunable sensitivity, along with eforts to combine CA 19–9 with other biomarkers for improved accuracy, open up the possibility for multiplexed NIR-fuorescence enhancement microarrays, for early cancer diagnosis and therapeutic monitoring.
Issue Date: 30-Oct-2017
Date of Acceptance: 11-Oct-2017
URI: http://hdl.handle.net/10044/1/51822
DOI: https://dx.doi.org/10.1038/s41598-017-14688-z
ISSN: 2045-2322
Publisher: Nature Publishing Group
Journal / Book Title: Scientific Reports
Volume: 7
Copyright Statement: This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. © The Author(s) 2017
Sponsor/Funder: British Council (UK)
Funder's Grant Number: 216239013
Publication Status: Published
Article Number: 14309
Appears in Collections:Materials
Department of Surgery and Cancer
Faculty of Engineering