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In vivo multiphoton microscopy using a handheld scanner with lateral and axial motion compensation

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Title: In vivo multiphoton microscopy using a handheld scanner with lateral and axial motion compensation
Authors: Sherlock, B
Warren, SC
Alexandrov, Y
Yu, F
Stone, J
Knight, J
Neil, MAA
Paterson, C
French, PMW
Dunsby, CW
Item Type: Journal Article
Abstract: This paper reports a handheld multiphoton fluorescence microscope designed for clinical imaging that incorporates axial motion compensation and lateral image stabilization. Spectral domain optical coherence tomography is employed to track the axial position of the skin surface, and lateral motion compensation is realised by imaging the speckle pattern arising from the optical coherence tomography beam illuminating the sample. Our system is able to correct lateral sample velocities of up to ~65 μm s-1. Combined with the use of negative curvature microstructured optical fibre to deliver tunable ultrafast radiation to the handheld multiphoton scanner without the need of a dispersion compensation unit, this instrument has potential for a range of clinical applications. The system is used to compensate for both lateral and axial motion of the sample when imaging human skin in vivo.
Issue Date: 31-Aug-2017
Date of Acceptance: 28-Aug-2017
URI: http://hdl.handle.net/10044/1/50536
DOI: https://dx.doi.org/10.1002/jbio.201700131
ISSN: 1864-063X
Publisher: Wiley-VCH Verlag
Journal / Book Title: Journal of Biophotonics
Volume: 11
Issue: 2
Copyright Statement: © 2017 The Authors. Journal of Biophotonics published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Sponsor/Funder: Engineering & Physical Science Research Council (EPSRC)
Funder's Grant Number: EP/K020102/1
Keywords: autofluorescence
motion compensation
Optoelectronics & Photonics
Publication Status: Published
Article Number: e201700131
Appears in Collections:Physics
Faculty of Natural Sciences