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Rhinovirus induction of fractalkine (CX3CL1) in airway and peripheral blood mononuclear cells in asthma

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Title: Rhinovirus induction of fractalkine (CX3CL1) in airway and peripheral blood mononuclear cells in asthma
Authors: Upton, N
Jackson, DJ
Nikonova, AA
Hingley-Wilson, S
Khaitov, M
Del Rosario, A
Traub, S
Trujillo-Torralbo, MB
Habibi, M
Elkin, SL
Kon, OM
Edwards, MR
Mallia, P
Footitt, J
MacIntyre, J
Stanciu, LA
Johnston, SL
Sykes, A
Item Type: Journal Article
Abstract: Rhinovirus infection is associated with the majority of asthma exacerbations. The role of fractalkine in anti-viral (type 1) and pathogenic (type 2) responses to rhinovirus infection in allergic asthma is unknown. To determine whether (1) fractalkine is produced in airway cells and in peripheral blood leucocytes, (2) rhinovirus infection increases production of fractalkine and (3) levels of fractalkine differ in asthmatic compared to non-asthmatic subjects. Fractalkine protein and mRNA levels were measured in bronchoalveolar lavage (BAL) cells and peripheral blood mononuclear cells (PBMCs) from non-asthmatic controls (n = 15) and mild allergic asthmatic (n = 15) subjects. Protein levels of fractalkine were also measured in macrophages polarised ex vivo to give M1 (type 1) and M2 (type 2) macrophages and in BAL fluid obtained from mild (n = 11) and moderate (n = 14) allergic asthmatic and non-asthmatic control (n = 10) subjects pre and post in vivo rhinovirus infection. BAL cells produced significantly greater levels of fractalkine than PBMCs. Rhinovirus infection increased production of fractalkine by BAL cells from non-asthmatic controls (P<0.01) and in M1-polarised macrophages (P<0.05), but not in BAL cells from mild asthmatics or in M2 polarised macrophages. Rhinovirus induced fractalkine in PBMCs from asthmatic (P<0.001) and healthy control subjects (P<0.05). Trends towards induction of fractalkine in moderate asthmatic subjects during in vivo rhinovirus infection failed to reach statistical significance. Fractalkine may be involved in both immunopathological and anti-viral immune responses to rhinovirus infection. Further investigation into how fractalkine is regulated across different cell types and into the effect of stimulation including rhinovirus infection is warranted to better understand the precise role of this unique dual adhesion factor and chemokine in immune cell recruitment.
Issue Date: 31-Aug-2017
Date of Acceptance: 2-Aug-2017
URI: http://hdl.handle.net/10044/1/50333
DOI: https://dx.doi.org/10.1371/journal.pone.0183864
ISSN: 1932-6203
Publisher: Public Library of Science (PLoS)
Journal / Book Title: PLoS ONE
Volume: 12
Issue: 8
Copyright Statement: © 2017 Upton et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Sponsor/Funder: Medical Research Council (MRC)
Commission of the European Communities
Janssen Biotech Inc
Commission of the European Communities
Asthma UK
Asthma UK
Medical Research Council (MRC)
National Institute for Health Research
Funder's Grant Number: G0601236
Keywords: MD Multidisciplinary
General Science & Technology
Publication Status: Published
Article Number: e0183864
Appears in Collections:National Heart and Lung Institute