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Role of PatS and cell type on the heterocyst spacing pattern in a filamentous branching cyanobacterium

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Title: Role of PatS and cell type on the heterocyst spacing pattern in a filamentous branching cyanobacterium
Authors: Antonaru, LA
Nuernberg, DJ
Item Type: Journal Article
Abstract: Cell differentiation is one of the marks of multicellular organisms. Terminally specialised nitrogen-fixing cells, termed heterocysts, evolved in filamentous cyanobacteria more than 2 Gya. The development of their spacing pattern has been thoroughly investigated in model organisms such as Anabaena sp. PCC 7120. This paper focuses on the more complex, branching cyanobacterium Mastigocladus laminosus (Stigonematales). Contrary to what has been previously published, a heterocyst spacing pattern is present in M. laminosus but it varies with the age of the culture and the morphology of the cells. Heterocysts in young, narrow trichomes were more widely spaced (∼14.8 cells) than those in old, wide trichomes (∼9.4 cells). Biochemical and transgenic experiments reveal that the heterocyst spacing pattern is affected by the heterocyst inhibitor PatS. Addition of the pentapeptide RGSGR (PatS-5) to the growth medium and overexpression of patS from Anabaena sp. PCC 7120 in M. laminosus resulted in the loss of heterocyst differentiation under nitrogen deprivation. Bioinformatics investigations indicated that putative PatS sequences within cyanobacteria are highly diverse, and fall into two main clades. Both are present in most branching cyanobacteria. Despite its more complex, branching phenotype, M. laminosus appears to use a PatS-based pathway for heterocyst differentiation, a property shared by Anabaena/Nostoc.
Issue Date: 21-Jul-2017
Date of Acceptance: 14-Jul-2017
URI: http://hdl.handle.net/10044/1/50130
DOI: https://dx.doi.org/10.1093/femsle/fnx154
ISSN: 0378-1097
Publisher: Oxford University Press (OUP)
Journal / Book Title: FEMS Microbiology Letters
Volume: 364
Issue: 15
Copyright Statement: © FEMS 2017. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
Keywords: 06 Biological Sciences
11 Medical And Health Sciences
07 Agricultural And Veterinary Sciences
Publication Status: Published
Article Number: fnx154
Appears in Collections:Faculty of Natural Sciences