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Nanopore extended field effect transistor for selective single molecule biosensing

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Title: Nanopore extended field effect transistor for selective single molecule biosensing
Authors: Ren, R
Zhang, Y
Paulose Nadappuram, B
Akpinar, B
Klenerman, D
Ivanov, AP
Edel, JB
Korchev, Y
Item Type: Journal Article
Abstract: There has been a significant drive to deliver nanotechnological solutions to biosensing, yet there remains an unmet need in the development of biosensors that are affordable, integrated, fast, capable of multiplexed detection, and offer high selectivity for trace analyte detection in biological fluids. Herein, some of these challenges are addressed by designing a new class of nanoscale sensors dubbed nanopore extended field-effect transistor (nexFET) that combine the advantages of nanopore single-molecule sensing, field-effect transistors, and recognition chemistry. We report on a polypyrrole functionalized nexFET, with controllable gate voltage that can be used to switch on/off, and slow down single-molecule DNA transport through a nanopore. This strategy enables higher molecular throughput, enhanced signal-to-noise, and even heightened selectivity via functionalization with an embedded receptor. This is shown for selective sensing of an anti-insulin antibody in the presence of its IgG isotype.
Issue Date: 19-Sep-2017
Date of Acceptance: 11-Jul-2017
URI: http://hdl.handle.net/10044/1/50106
DOI: 10.1038/s41467-017-00549-w
ISSN: 2041-1723
Publisher: Nature Publishing Group
Start Page: 1
End Page: 9
Journal / Book Title: Nature Communications
Volume: 8
Copyright Statement: This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/ licenses/by/4.0/. © The Author(s) 2017
Sponsor/Funder: Commission of the European Communities
Engineering & Physical Science Research Council (EPSRC)
Commission of the European Communities
Commission of the European Communities
Biotechnology and Biological Sciences Research Council (BBSRC)
Imperial College London
Engineering and Physical Sciences Research Council
Funder's Grant Number: 724300
EP/P011985/1
279818
677677
BB/L017865/1
EP/L015277/1
Keywords: Science & Technology
Multidisciplinary Sciences
Science & Technology - Other Topics
ELECTRONIC DETECTION
DNA
SENSORS
TRANSLOCATION
PROTEINS
DELIVERY
RECEPTOR
Biosensing Techniques
DNA
Insulin
Nanopores
Nanostructures
Nanotechnology
Sensitivity and Specificity
Insulin
DNA
Sensitivity and Specificity
Biosensing Techniques
Nanotechnology
Nanostructures
Nanopores
Publication Status: Published
Article Number: 586
Online Publication Date: 2017-09-19
Appears in Collections:Department of Metabolism, Digestion and Reproduction
Chemistry
Faculty of Medicine
Faculty of Natural Sciences