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N-glycosylation status of beta-haptoglobin in sera of patients with prostate cancer vs. benign prostate diseases

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Title: N-glycosylation status of beta-haptoglobin in sera of patients with prostate cancer vs. benign prostate diseases
Authors: Yoon, S-J
Park, S-Y
Pang, P-C
Gallagher, J
Gottesman, JE
Dell, A
Kim, J-H
Hakomori, S-I
Item Type: Journal Article
Abstract: N-glycosylation status of purified β-haptoglobin separated from sera of patients with prostate cancer was studied in comparison to that of sera from patients with benign prostate diseases, or normal subjects. Two different approaches, as summarized below, one based on binding of lectins and antibodies to β-haptoglobin, the other on mass spectrometry of released N-linked glycans from β-haptoglobin, were performed. Some of the results were useful for distinction of prostate cancer vs. benign prostate diseases. i) Binding of Phaseolus vulgaris-L lectin (PHA-L), defining the GlcNAcβ6Manα6Man side chain present in tri- or tetra-antennary N-linked glycans, to β-haptoglobin was higher for cases of prostate cancer and high-grade prostate intraepithelial neoplasia than for benign diseases. Binding of Aleuria aurantia lectin (AAL) defining Fucα3-, α4-, or α6-GlcNAc, or monoclonal antibody directed to sialyl-Lex, to β-haptoglobin was also higher for some of the cancer cases than for benign diseases. Many other lectins and antibodies showed no binding to β-haptoglobin, or showed no significant difference between cancer vs. benign diseases. ii) Mass spectrometric analysis of N-linked glycans of β-haptoglobin released by Peptide N-glycosidase-F showed enhanced expression of monosialyl tri-antennary structures in prostate cancer cases. Thus, binding of PHA-L to affinity-purified β-haptoglobin from sera of patients could lead to development of useful tools for differential diagnosis of prostate cancer vs. benign prostate diseases.
Issue Date: 1-Jan-2010
Date of Acceptance: 28-Jul-2009
URI: http://hdl.handle.net/10044/1/49847
DOI: https://dx.doi.org/10.3892/ijo_00000490
ISSN: 1019-6439
Publisher: Spandidos Publications
Start Page: 193
End Page: 203
Journal / Book Title: International Journal of Oncology
Volume: 36
Issue: 1
Copyright Statement: © 2010 Spandidos Publications UK Ltd. Original source: Yoon, S., Park, S., Pang, P., Gallagher, J., Gottesman, J. E., Dell, A., Kim, J., Hakomori, S."N-glycosylation status of β-haptoglobin in sera of patients with prostate cancer vs. benign prostate diseases". International Journal of Oncology 36.1 (2010): 193-203.
Sponsor/Funder: Biotechnology and Biological Sciences Research Council (BBSRC)
Biotechnology and Biological Sciences Research Council (BBSRC)
Funder's Grant Number: SF19107
BB/F008309/1
Keywords: Science & Technology
Life Sciences & Biomedicine
Oncology
ONCOLOGY
haptoglobin
prostate cancer
lectin blotting
N-linked glycan
mass spectrometry
ACETYLGLUCOSAMINYLTRANSFERASE-V
TRANSCRIPTIONAL REGULATION
CARBOHYDRATE ANTIGENS
PANCREATIC-CANCER
TUMOR MALIGNANCY
VACCINES
GANGLIOSIDE
ANTIBODIES
CARCINOMA
MARKERS
Aged
Chromatography
Enzyme-Linked Immunosorbent Assay
Epitopes
Glycosylation
Haptoglobins
Humans
Lectins
Male
Mass Spectrometry
Middle Aged
Phaseolus
Prostatic Diseases
Prostatic Neoplasms
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
1112 Oncology And Carcinogenesis
Oncology & Carcinogenesis
Publication Status: Published
Appears in Collections:Faculty of Natural Sciences