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Microbiota-induced peritrophic matrix regulates midgut homeostasis and prevents systemic infection of malaria vector mosquitoes

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Title: Microbiota-induced peritrophic matrix regulates midgut homeostasis and prevents systemic infection of malaria vector mosquitoes
Authors: Rodgers, FH
Gendrin, M
Wyer, CAS
Christophides, GK
Item Type: Journal Article
Abstract: Manipulation of the mosquito gut microbiota can lay the foundations for novel methods for disease transmission control. Mosquito blood feeding triggers a significant, transient increase of the gut microbiota, but little is known about the mechanisms by which the mosquito controls this bacterial growth whilst limiting inflammation of the gut epithelium. Here, we investigate the gut epithelial response to the changing microbiota load upon blood feeding in the malaria vector Anopheles coluzzii. We show that the synthesis and integrity of the peritrophic matrix, which physically separates the gut epithelium from its luminal contents, is microbiota dependent. We reveal that the peritrophic matrix limits the growth and persistence of Enterobacteriaceae within the gut, whilst preventing seeding of a systemic infection. Our results demonstrate that the peritrophic matrix is a key regulator of mosquito gut homeostasis and establish functional analogies between this and the mucus layers of the mammalian gastrointestinal tract.
Issue Date: 1-May-2017
Date of Acceptance: 28-Apr-2017
URI: http://hdl.handle.net/10044/1/48855
DOI: 10.1371/journal.ppat.1006391
ISSN: 1553-7366
Publisher: Public Library of Science
Start Page: 1
End Page: 22
Journal / Book Title: PLOS Pathogens
Volume: 13
Issue: 5
Copyright Statement: © 2017 Rodgers et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited (https://creativecommons.org/licenses/by/4.0/)
Sponsor/Funder: Commission of the European Communities
Biotechnology and Biological Sciences Research Council (BBSRC)
Funder's Grant Number: 223736
BB/k009338/1
Keywords: Science & Technology
Life Sciences & Biomedicine
Microbiology
Parasitology
Virology
ANOPHELES-GAMBIAE
BACTERIAL TRANSLOCATION
ANTIBACTERIAL RESPONSE
PARASITE CHITINASE
GUT MICROBIOTA
PLASMODIUM
GENE
PROTEIN
IDENTIFICATION
PATHWAY
Animals
Anopheles
Epithelium
Female
Gastrointestinal Tract
Gene Library
Homeostasis
Host-Parasite Interactions
Humans
Malaria
Microbiota
Mosquito Vectors
Sepsis
Sequence Analysis, RNA
Gastrointestinal Tract
Epithelium
Animals
Humans
Anopheles
Sepsis
Malaria
Sequence Analysis, RNA
Homeostasis
Gene Library
Female
Host-Parasite Interactions
Microbiota
Mosquito Vectors
Science & Technology
Life Sciences & Biomedicine
Microbiology
Parasitology
Virology
ANOPHELES-GAMBIAE
GUT MICROBIOTA
AEDES-AEGYPTI
BACTERIAL TRANSLOCATION
DROSOPHILA-MELANOGASTER
ANTIBACTERIAL RESPONSE
PARASITE CHITINASE
BINDING-PROTEIN
PLASMODIUM
GENE
Virology
0605 Microbiology
1107 Immunology
1108 Medical Microbiology
Publication Status: Published
Article Number: ARTN e1006391
Online Publication Date: 2017-05-17
Appears in Collections:Faculty of Natural Sciences