IRUS Total

Multiplexed single cell protein expression analysis in solid tumours using a miniaturised microfluidic assay

File Description SizeFormat 
Magness_2017_Converg._Sci._Phys._Oncol._3_024003.pdfPublished version2.41 MBAdobe PDFView/Open
Title: Multiplexed single cell protein expression analysis in solid tumours using a miniaturised microfluidic assay
Authors: Magness, AJ
Squires, J
Griffiths, B
Khan, K
Swain, A
Willison, K
Cunningham, D
Gerlinger, M
Klug, D
Item Type: Journal Article
Abstract: Using patient-derived colorectal cancer xenografts, we demonstrate a practicable workflow for single cell proteomics in clinically relevant samples and thus a potential translational route for single cell proteomics into medical diagnostics. Using a microfluidic antibody capture [MAC] chip we measured the expression of the tumour suppressor protein p53 and of its post-translationally modified form phosphorylated at serine-15. Aberrant expression of these has commonly been found in colorectal cancers and has been widely investigated for prognostic significance. Our results show that the MAC technology is viable for quantitatively assessing protein expression and phosphorylation at the single cell level in microscopic amounts of clinically relevant tumour material. Thus, this could become a useful tool in therapeutic-associated single cell protein analysis. We also found dramatic variability of p53 and phosphorylated p53 quantities between individual cancer cells from the same sample, demonstrating the power of this single cell technology to study functional intratumour heterogeneity.
Issue Date: 28-Apr-2017
Date of Acceptance: 3-Apr-2017
URI: http://hdl.handle.net/10044/1/46253
DOI: https://dx.doi.org/10.1088/2057-1739/aa6aae
ISSN: 2057-1739
Publisher: IOP Publishing
Journal / Book Title: Convergent Science Physical Oncology
Volume: 3
Issue: 2
Copyright Statement: © 2017 IOP Publishing Ltd. Original content from this work may be used under the terms of the Creative Commons Attribution 3.0 licence. Any further distribution of this work must maintain attribution to the author(s) and the title of the work, journal citation and DOI.
Sponsor/Funder: Engineering & Physical Science Research Council (E
Engineering & Physical Science Research Council (EPSRC)
Commission of the European Communities
Funder's Grant Number: EP/I017887/1
Publication Status: Published
Article Number: 0240 03
Appears in Collections:Chemistry
Biological and Biophysical Chemistry
National Heart and Lung Institute
Faculty of Natural Sciences