32
IRUS Total
Downloads
  Altmetric

Monomeric, porous type II collagen scaffolds promote chondrogenic differentiation of human bone marrow mesenchymal stem cells in vitro

File Description SizeFormat 
srep43519.pdfPublished version1.45 MBAdobe PDFView/Open
Title: Monomeric, porous type II collagen scaffolds promote chondrogenic differentiation of human bone marrow mesenchymal stem cells in vitro
Authors: Tamaddon, M
Burrows, M
Ferreira, SA
Dazzi, F
Apperley, JF
Bradshaw, A
Brand, DD
Czernuszka, J
Gentleman, E
Item Type: Journal Article
Abstract: Osteoarthritis (OA) is a common cause of pain and disability and is often associated with the degeneration of articular cartilage. Lesions to the articular surface, which are thought to progress to OA, have the potential to be repaired using tissue engineering strategies; however, it remains challenging to instruct cell differentiation within a scaffold to produce tissue with appropriate structural, chemical and mechanical properties. We aimed to address this by driving progenitor cells to adopt a chondrogenic phenotype through the tailoring of scaffold composition and physical properties. Monomeric type-I and type-II collagen scaffolds, which avoid potential immunogenicity associated with fibrillar collagens, were fabricated with and without chondroitin sulfate (CS) and their ability to stimulate the chondrogenic differentiation of human bone marrow-derived mesenchymal stem cells was assessed. Immunohistochemical analyses showed that cells produced abundant collagen type-II on type-II scaffolds and collagen type-I on type-I scaffolds. Gene expression analyses indicated that the addition of CS – which was released from scaffolds quickly – significantly upregulated expression of type II collagen, compared to type-I and pure type-II scaffolds. We conclude that collagen type-II and CS can be used to promote a more chondrogenic phenotype in the absence of growth factors, potentially providing an eventual therapy to prevent OA.
Issue Date: 3-Mar-2017
Date of Acceptance: 25-Jan-2017
URI: http://hdl.handle.net/10044/1/45888
DOI: https://dx.doi.org/10.1038/srep43519
ISSN: 2045-2322
Publisher: NATURE PUBLISHING GROUP
Journal / Book Title: SCIENTIFIC REPORTS
Volume: 7
Copyright Statement: © 2017 The Author(s). This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
Sponsor/Funder: Medical Research Council (MRC)
Bloodwise
Kay Kendall Leukemia Fund
Leukaemia & Lymphoma Research "Beating Blood Cancers"
Leuka
Fundação para a Ciência e a Tecnologia
Leuka
National Institute for Health Research
Funder's Grant Number: MC_G0802523
10001
KKL444
11026
WMEH_P43080
WMEH_P43267
n/a
NF-SI-0611-10275
Keywords: Science & Technology
Multidisciplinary Sciences
Science & Technology - Other Topics
ARTICULAR-CARTILAGE REPAIR
CHONDROITIN-SULFATE
EXTRACELLULAR-MATRIX
OSTEOGENIC DIFFERENTIATION
3-DIMENSIONAL SCAFFOLDS
MECHANICAL-PROPERTIES
PORE-SIZE
REGENERATION
CHONDROCYTES
BEHAVIOR
Publication Status: Published
Article Number: ARTN 43519
Appears in Collections:Department of Medicine (up to 2019)