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Adenosine preconditioning attenuates hepatic reperfusion injury in the rat by preventing the down-regulation of endothelial nitric oxide synthase.

Title: Adenosine preconditioning attenuates hepatic reperfusion injury in the rat by preventing the down-regulation of endothelial nitric oxide synthase.
Authors: Serracino-Inglott, F
Virlos, IT
Habib, NA
Williamson, RC
Mathie, RT
Item Type: Journal Article
Abstract: BACKGROUND: Previous work has suggested that in the liver, adenosine preconditioning is mediated by nitric oxide. Whether the endothelial isoform of nitric oxide synthase plays a part in this mechanism has however not yet been investigated. METHODS: Wistar rats were used (6 in each group)--Groups: (1) sham, (2) ischemia-reperfusion, (3) adenosine + ischemia-reperfusion, (4) endothelial isoform inhibitor + adenosine + ischemia-reperfusion. RESULTS: Using immunohistochemistry, this study has revealed a decrease in the expression of endothelial nitric oxide synthase following hepatic ischemia-reperfusion. This was prevented by adenosine pre-treatment. When an inhibitor of endothelial nitric oxide synthase was administered prior to adenosine pre-treatment, pre-conditioning did not occur despite normal expression of endothelial nitric oxide synthase. CONCLUSIONS: These findings suggest that adenosine attenuates hepatic injury by preventing the downregulation of endothelial nitric oxide synthase that occurs during ischemia-reperfusion.
Issue Date: 20-Sep-2002
URI: http://hdl.handle.net/10044/1/45635
DOI: https://dx.doi.org/10.1186/1471-230X-2-22
Start Page: 22
Journal / Book Title: BMC Gastroenterol
Volume: 2
Copyright Statement: © 2002 Serracino-Inglott et al; licensee BioMed Central Ltd. This article is published under license to BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.
Keywords: Adenosine
Alanine Transaminase
Animals
Aspartate Aminotransferases
Down-Regulation
Ischemia
Ischemic Preconditioning
Liver
Liver Diseases
Male
Nitric Oxide Synthase
Rats
Rats, Wistar
Reperfusion Injury
Gastroenterology & Hepatology
1103 Clinical Sciences
Publication Status: Published
Conference Place: England
Appears in Collections:Department of Surgery and Cancer