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Imaging tumor microscopic viscosity in vivo using molecular rotors

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Title: Imaging tumor microscopic viscosity in vivo using molecular rotors
Authors: Shimolina, LE
Izquierdo, MA
Lopez-Duarte, I
Bull, JA
Shirmanova, MV
Klapshina, LG
Zagaynova, EV
Kuimova, MK
Item Type: Journal Article
Abstract: The microscopic viscosity plays an essential role in cellular biophysics by controlling the rates of diffusion and bimolecular reactions within the cell interior. While several approaches have emerged that have allowed the measurement of viscosity and diffusion on a single cell level in vitro, the in vivo viscosity monitoring has not yet been realized. Here we report the use of fluorescent molecular rotors in combination with Fluorescence Lifetime Imaging Microscopy (FLIM) to image microscopic viscosity in vivo, both on a single cell level and in connecting tissues of subcutaneous tumors in mice. We find that viscosities recorded from single tumor cells in vivo correlate well with the in vitro values from the same cancer cell line. Importantly, our new method allows both imaging and dynamic monitoring of viscosity changes in real time in live animals and thus it is particularly suitable for diagnostics and monitoring of the progress of treatments that might be accompanied by changes in microscopic viscosity.
Issue Date: 30-Jan-2017
Date of Acceptance: 12-Dec-2016
URI: http://hdl.handle.net/10044/1/44735
DOI: http://dx.doi.org/10.1038/srep41097
ISSN: 2045-2322
Publisher: Nature Publishing Group
Journal / Book Title: Scientific Reports
Volume: 7
Copyright Statement: © 2017 The Authors. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
Sponsor/Funder: Engineering & Physical Science Research Council (EPSRC)
Commission of the European Communities
The Royal Society
Funder's Grant Number: EP/I003983/1
327334
UF140161
Keywords: Science & Technology
Multidisciplinary Sciences
Science & Technology - Other Topics
FLUORESCENCE CORRELATION SPECTROSCOPY
LIVE CELLS
PHOTODYNAMIC THERAPY
CELLULAR-VISCOSITY
PLASMA-MEMBRANES
PHASE-SEPARATION
INNER MEMBRANE
ACID) BRUSHES
LIVING CELLS
SINGLE-CELL
Publication Status: Published
Article Number: ARTN 41097
Appears in Collections:Chemistry
Faculty of Natural Sciences



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