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Quantitative volumetric Raman imaging of three dimensional cell cultures

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Title: Quantitative volumetric Raman imaging of three dimensional cell cultures
Authors: Kallepitis, C
Bergholt, MS
Mazo, MM
Leonardo, V
Skaalure, SC
Maynard, SA
Stevens, MM
Item Type: Journal Article
Abstract: The ability to simultaneously image multiple biomolecules in biologically relevant three-dimensional (3D) cell culture environments would contribute greatly to the understanding of complex cellular mechanisms and cell-material interactions. Here, we present a computational framework for label-free quantitative volumetric Raman imaging (qVRI). We apply qVRI to a selection of biological systems: human pluripotent stem cells with their cardiac derivatives, monocytes and monocyte-derived macrophages in conventional cell culture systems and mesenchymal stem cells inside biomimetic hydrogels that supplied a 3D cell culture environment. We demonstrate visualization and quantification of fine details in 3D cell shape, cytoplasm, nucleus, lipid bodies and cytoskeletal structures in 3D with unprecedented biomolecular specificity for vibrational microspectroscopy.
Issue Date: 22-Mar-2017
Date of Acceptance: 15-Jan-2017
URI: http://hdl.handle.net/10044/1/44038
DOI: http://dx.doi.org/10.1038/ncomms14843
ISSN: 2041-1723
Publisher: Nature Publishing Group
Journal / Book Title: Nature Communications
Volume: 8
Copyright Statement: © The Author(s) 2017. This work is licensed under a Creative Commons Attribution 4.0 International License.
Sponsor/Funder: Medical Research Council (MRC)
Medical Research Council (MRC)
Wellcome Trust
Commission of the European Communities
Commission of the European Communities
Funder's Grant Number: MR/K026666/1
Keywords: MD Multidisciplinary
Publication Status: Published
Article Number: 14843
Appears in Collections:Materials
Faculty of Natural Sciences
Faculty of Engineering