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Modulation of the molecular arrangement in artificial and biological membranes by phospholipid-shelled microbubbles

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Title: Modulation of the molecular arrangement in artificial and biological membranes by phospholipid-shelled microbubbles
Authors: Carugo, D
Aron, M
Sezgin, E
Bernardino de la Serna, J
Kuimova, MK
Eggeling, C
Stride, E
Item Type: Journal Article
Abstract: The transfer of material from phospholipid-coated microbubbles to cell membranes has been hypothesized to play a role in ultrasound-mediated drug delivery. In this study, we employed quantitative fluorescence microscopy techniques to investigate this phenomenon in both artificial and biological membrane bilayers in an acoustofluidic system. The results of the present study provide strong evidence for the transfer of material from microbubble coatings into cell membranes. Our results indicate that transfer of phospholipids alters the organization of molecules in cell membranes, specifically the lipid ordering or packing, which is known to be a key determinant of membrane mechanical properties, protein dynamics, and permeability. We further show that polyethylene-glycol, used in many clinical microbubble formulations, also has a major impact on both membrane lipid ordering and the extent of lipid transfer, and that this occurs even in the absence of ultrasound exposure.
Issue Date: 24-Oct-2016
Date of Acceptance: 23-Oct-2016
URI: http://hdl.handle.net/10044/1/42813
DOI: http://dx.doi.org/10.1016/j.biomaterials.2016.10.034
ISSN: 1878-5905
Publisher: Elsevier
Start Page: 105
End Page: 117
Journal / Book Title: Biomaterials
Volume: 113
Copyright Statement: © 2016 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
Sponsor/Funder: Engineering & Physical Science Research Council (EPSRC)
Funder's Grant Number: EP/I003983/1
Keywords: Lipid order
Membrane bilayers
Microbubbles
Ultrasound
Biomedical Engineering
MD Multidisciplinary
Publication Status: Published
Conference Place: Netherlands
Appears in Collections:Chemistry
Faculty of Natural Sciences



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