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Structures of the RNA polymerase-sigma(54) reveal new and conserved regulatory strategies

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Title: Structures of the RNA polymerase-sigma(54) reveal new and conserved regulatory strategies
Authors: Yang, Y
Darbari, VC
Zhang, N
Lu, D
Glyde, R
Wang, Y-P
Winkelman, JT
Gourse, RL
Murakami, KS
Buck, M
Zhang, X
Item Type: Journal Article
Abstract: Transcription by RNA polymerase (RNAP) in bacteria requires specific promoter recognition by σ factors. The major variant σ factor (σ54) initially forms a transcriptionally silent complex requiring specialized adenosine triphosphate–dependent activators for initiation. Our crystal structure of the 450-kilodalton RNAP-σ54 holoenzyme at 3.8 angstroms reveals molecular details of σ54 and its interactions with RNAP. The structure explains how σ54 targets different regions in RNAP to exert its inhibitory function. Although σ54 and the major σ factor, σ70, have similar functional domains and contact similar regions of RNAP, unanticipated differences are observed in their domain arrangement and interactions with RNAP, explaining their distinct properties. Furthermore, we observe evolutionarily conserved regulatory hotspots in RNAPs that can be targeted by a diverse range of mechanisms to fine tune transcription.
Issue Date: 21-Aug-2015
Date of Acceptance: 15-Jul-2015
URI: http://hdl.handle.net/10044/1/42431
DOI: https://dx.doi.org/10.1126/science.aab1478
ISSN: 0036-8075
Publisher: American Association for the Advancement of Science
Start Page: 882
End Page: 885
Journal / Book Title: Science
Volume: 349
Issue: 6250
Copyright Statement: © 2016 the American Association for the Advancement of Science; all rights reserved. This is the author’s version of the work. It is posted here by permission of the AAAS for personal use, not for redistribution. The definitive version was published in Science on 21 August 2015 in Volume 349, DOI: https://dx.doi.org/10.1126/science.aab1478
Sponsor/Funder: Biotechnology and Biological Sciences Research Council (BBSRC)
Biotechnology and Biological Sciences Research Council (BBSRC)
Funder's Grant Number: BB/C504700/1
BB/J00717X/1
Keywords: Science & Technology
Multidisciplinary Sciences
Science & Technology - Other Topics
II-TFIIB COMPLEX
TRANSCRIPTION INITIATION
ESCHERICHIA-COLI
SINGLE-MOLECULE
BACTERIAL
SIGMA
DNA
POLYMERASES
DOMAIN
HOLOENZYME
Crystallography, X-Ray
Enzyme Stability
Evolution, Molecular
Gene Expression Regulation
Holoenzymes
Protein Conformation
Protein Structure, Tertiary
RNA Polymerase Sigma 54
Transcription, Genetic
General Science & Technology
MD Multidisciplinary
Publication Status: Published
Appears in Collections:Department of Medicine (up to 2019)
Faculty of Natural Sciences